Federal University of Pelotas 2 articles published in JoVE Immunology and Infection Experimental Model of Ligature-Induced Peri-Implantitis in Mice Davi Neto de Araújo Silva1, Maísa Casarin2, Sepehr Monajemzadeh1, Taciane Menezes da Silveira2, Jacob Lubben1, Beatriz Bezerra1, Flavia Q. Pirih1 1School of Dentistry, Section of Periodontics, University of California, 2School of Dentistry, Department of Periodontology, Federal University of Pelotas This is a report on an experimental model of ligature-induced peri-implantitis in mice. We describe all surgical steps, from pre- and post-operative management of the animals, extractions, implant placement, and ligature-induced peri-implantitis. Biology Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome Sarah R. Ocañas*1,2,3, José V. V. Isola*1,3, Tatiana D. Saccon1, Kevin D. Pham2, Ana J. Chucair-Elliott2, Augusto Schneider4, Willard M. Freeman2,3, Michael B. Stout1,3 1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas In this protocol, the translating ribosome affinity purification (TRAP) method and the isolation of nuclei tagged in specific cell types (INTACT) method were optimized for the paired interrogation of the cell-specific ovarian transcriptome and epigenome using the NuTRAP mouse model crossed to a Cyp17a1-Cre mouse line.
