Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Studying the Effects of Matrix Stiffness on…
JoVE Journal
Biology

É necessário ter uma assinatura JoVE para assistir este

JoVE Journal Biology
Studying the Effects of Matrix Stiffness on Cellular Function using Acrylamide-based Hydrogels
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
Journal (Biology)
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
Journal (Biology)
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
 

Studying the Effects of Matrix Stiffness on Cellular Function using Acrylamide-based Hydrogels

Article DOI: 10.3791/2089-v • 10:20 min • August 10th, 2010
August 10th, 2010 •
1, 1, 1
1Department of Pharmacology, University of Pennsylvania

Capítulos

Resumo
Automatic Translation

The effect of substrata stiffness on cellular function can be modeled in vitro using polyacrylamide hydrogels of varying compliances.

Tags

Matrix Stiffness Cellular Function Acrylamide-based Hydrogels Tissue Stiffness Fibrosis Cancer Cardiovascular Disease Cell Biology ECM-coated Hydrogels Polyacrylamide NaOH 3-APTMS Glutaraldehyde Acrylamide (AC) Bis-acrylamide (Bis-AC) Ammonium Persulfate N-hydroxysuccinimide (NHS) Rheology Atomic Force Microscopy (AFM)
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter