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Immunology and Infection

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JoVE Journal Immunology and Infection
Alphavirus Transducing System
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Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors

Article DOI: 10.3791/2363-v • 07:12 min • November 24th, 2010
November 24th, 2010 •
1, 1, 1, 1, 1
1Microbiology, Immunology, and Pathology, Colorado State University

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Methods for using alphavirus transducing systems to express fluorescent reporters in vitro and in adult mosquitoes are described. This technique may be adapted to express any protein of interest in lieu of or in addition to a reporter.

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Alphavirus Transducing System Visualizing Infection Mosquito Vectors Gene Expression Mosquito-borne RNA Viruses Alphavirus Genus CDNA Clones Single-stranded RNA Genomes Structural Proteins Gene Insertion Viral Subgenomic RNA Initiation Site Promoter Stable Protein Expression RNAi Gene Silencing
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