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A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
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A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Article DOI: 10.3791/2461-v • 10:22 min • February 1st, 2011
February 1st, 2011 •
1, 1, 1
1Microbiology and Immunology, University of British Columbia - UBC

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This protocol describes a high throughput screen for cellulolytic activity from a metagenomic library expressed in Escherichia coli. The screen is solution based and highly automated, and uses one-pot chemistry in 384 well microplates with the final readout as an absorbance measurement.

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High Throughput Screen Biomining Cellulase Activity Metagenomic Libraries Cellulose Industrial Applications Biofuel Production Chemical Methods Enzymatic Methods Cellulases Bacterial Isolates Fungal Isolates Laboratory Cultivation Environmental Genomic Screening Metagenomic Screening Approaches Soils Buffalo Rumen Termite Hind-gut Carboxymethylcellulose (CMC) Agar Plates Congo Red Dye Method Of Teather And Wood Signal To Noise Ratio Chromogenic Dinitrophenol (DNP)-cellobioside Substrate PCC1 C
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