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Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
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Capítulos
- 00:05Título
- 02:06Preparation of DNA and Glass Micropipettes for Injection
- 02:57Laparotomy
- 04:33DNA Injection into the Brain Ventricle
- 05:59Targeting the Hypothalamus for Electroporation
- 07:17Finishing the Surgery
- 08:28Analyzing the Results
- 09:18Results: Hypothalamus Transfection by In utero Electroporation
- 10:54Conclusion
Despite the functional and medical importance of the hypothalamus, in utero genetic manipulation of its development has rarely been attempted. We show a detailed procedure for in utero electroporation into the mouse hypothalamus and show representative results of total and partial (regional) hypothalamic transfection.
