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Journal / Biochemistry / 可溶性和不溶性杭丁顿蛋白的分离分解…
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JoVE Journal Biochemistry
可溶性和不溶性杭丁顿蛋白的分离分解
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可溶性和不溶性杭丁顿蛋白的分离分解

Article DOI: 10.3791/57082-v • 07:08 min • February 27th, 2018
February 27th, 2018 •
1,2, 2,3, 4, 1,2,3
1Department of Psychiatry and Human Behavior, University of California Irvine, 2UCI MIND, University of California Irvine, 3Department of Neurobiology and Behavior, University of California Irvine, 4Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center

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本文介绍了一种从小鼠脑和细胞培养中分离不溶性和可溶性突变体杭丁顿蛋白的方法。该方法对杭丁顿蛋白蛋白通量和艾滋病在分析疾病发病机制中蛋白质稳态和扰动存在时的定性和定量具有重要意义。

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生物化学 问题 132 亨廷顿氏病 聚集 杭丁顿蛋白 蛋白质分馏 不溶性 proteostasis
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