Journal
/
Bioquímica
/
Detection of Detergent-sensitive Interactions Between Membrane Proteins
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Detection of Detergent-sensitive Interactions Between Membrane Proteins

Detection of Detergent-sensitive Interactions Between Membrane Proteins

DOI:
10.3791/57179-v

•

10:09 min

•

March 07, 2018

•

, ,
1Department of Biochemistry,Boston University School of Medicine

Capítulos

  • 00:04Título
  • 01:05Handling of the Peptide and Cells
  • 03:02Binding of His-tagged Proteins to the HisPur Cobalt Beads
  • 04:39Binding of the Peptide to the His-tagged Protein Immobilized on the Beads
  • 05:59Elution from Cobalt Beads
  • 07:07Electrophoresis, Western Blotting, and Analysis of Results
  • 07:58Results: Use of Immunoblot Assay to Study the Interaction between Sortilin Immobilized on Cobalt Beads and myc-tagged Peptide
  • 09:18Conclusion

Summary

Tadução automática

Tadução automática

We describe a protocol for detection of detergent-sensitive interactions between membrane proteins using binding of the sorting receptor, sortilin, to the first luminal loop of the glucose transporter protein, GLUT4, as an example.

Tags

Membrane ProteinsDetergent-sensitive InteractionsHistidine-taggedMyc-taggedCo-immunoprecipitationCell Lysate3T3-L1 Pre-adipocytesTransfectionProtein InteractionsBioquímica

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs

Read Article