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In Vivo Zwei-Farben 2-Photon Enzgebung von genetisch markierten Reporterzellen in der Haut

Article DOI: 10.3791/59647-v • 05:45 min • July 11th, 2019
July 11th, 2019 •
1, 1, 1, 1
1School of Brain and Behavioral Science, Center for Advanced Pain Studies, University of Texas at Dallas

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Morphologische Veränderungen treten in immunreagierenden Fibroblastenzellen nach aktivierung auf und fördern Veränderungen bei der Zellrekrutierung. Verwendung von 2-Photonen-Bildgebung in Verbindung mit einem gentechnisch veränderten Fibroblasten-spezifischen Protein 1 (FSP1)-cre; tdTomato floxed-stop-floxed (TB/TB) Mauslinie und grün fluoreszierend markiert Lipopolysaccharid-FITC, können wir hochspezifische Aufnahme von Lipopolysaccharid in dermalen Fibroblasten und morphologischen Veränderungen in vivo veranschaulichen.

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Bioengineering Ausgabe 149 Multi-Photon Imaging toller Rezeptor 4 genetisch Reporter Lipopolysaccharid in vivo
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