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A Hydrophobic Tissue Clearing Method for Rat Brain Tissue
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Neurociência
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JoVE Journal Neurociência
A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

DOI:
10.3791/61821-v

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08:24 min

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December 23, 2020

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, , , , ,
1Program in Behavioral Neuroscience, Department of Psychology,University of South Carolina

Capítulos

  • 00:05Introduction
  • 00:46Sample Preparation
  • 01:36Dehydration and Depigmentation
  • 03:04CTB-Cholera Toxin Subunit B Labeling and Antibody Application
  • 04:17Tissue Clearing
  • 05:02Mounting the Sample for Imaging
  • 05:59Results: Confocal Images of the Hydrophobically Cleared Tissue Samples with Fluorescent Labelled TH Neurons and CTB Markers
  • 07:39Conclusion

Summary

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Here we present a hydrophobic tissue clearing method that allows for the viewing of target molecules as part of intact brain structures. This technique has now been validated for F344/N control and HIV-1 transgenic rats of both sexes.

Tags

Rat BrainHIV-1 Transgenic RatTranscardial PerfusionPFA FixationMethanol DehydrationDCM-methanol IncubationHydrogen Peroxide BleachingBiotin-CTB InjectionImmunostaining3D Imaging

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