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High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Article DOI: 10.3791/61913-v • 11:07 min • December 4th, 2021
December 4th, 2021 •
1,2, 1, 3, 3, 3, 3,4, 1, 1, 1,3
1Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi Research Institute, 2Department of Biology, United Arab Emirates University, 3Laboratory of Algal, Systems, and Synthetic Biology (LASSB), Division of Science and Math, New York University Abu Dhabi, 4Department of Marine Science, Ocean College, Zhejiang University

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This protocol demonstrates the use of a phenotype microarray (PM) technology platform to define metabolic requirements of Chlamydomonas reinhardtii, a green microalga, and refine an existing metabolic network model.

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High-throughput Metabolic Profiling Model Refinements Microalgae Phenotype Micro-array Technology Cellular Metabolic Activities Cellular Respiration Tetrazolium-based Redox Dye Phenotype Micro Array Assays Micro-algae Chlamydomonas Reinhardtii Metabolic Phenotyping Algal Metabolic Network Models Chlamydomonas Resource Center Tris-acetate Phosphate Fat Media Microscope Examination Contamination Check Centrifugation Tetrazolium Violet Dye Antibiotics Inhibition Bacterial Growth Inhibition
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