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JoVE Journal Biochemistry
Ortsspezifische Lysin-Acetylierung von Histonen zur Nukleosomen-Rekonstitution mit genetischer Codeerweiterung bei Escherichia coli
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Ortsspezifische Lysin-Acetylierung von Histonen zur Nukleosomen-Rekonstitution mit genetischer Codeerweiterung bei Escherichia coli

Article DOI: 10.3791/62113-v • 07:26 min • December 26th, 2020
December 26th, 2020 •
1, 1
1Department of Chemistry, Texas A&M University

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Hier präsentieren wir eine Methode, um acetylierte Histonproteine mittels genetischer Codeerweiterung auszudrücken und rekonstituierte Nukleosomen in vitro zusammenzusetzen.

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Biochemie Ausgabe 166 Nukleosom Acetylierung Pyrrolysin genetische Codeerweiterung posttranslationale Modifikationen Proteinmodifikationen Histonen
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