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One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster

Article DOI: 10.3791/64729-v • 07:23 min • January 26th, 2024
January 26th, 2024 •
*1, *2, 1,2,3
1Department of Cell and Developmental Biology, University of Michigan, 2Cellular and Molecular Biology Program, University of Michigan, 3Michigan Neuroscience Institute Affiliate, University of Michigan
* These authors contributed equally

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Here, we present a simplified endogenous gene tagging protocol for Drosophila, which utilizes a PCR-based technique for marker-free identification of successful genetic modifications, facilitating the development of stable knock-in lines.

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Neuroscience CRISPR endogenous gene tagging single-leg non-lethal PCR Drosophila
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