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Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes

Article DOI: 10.3791/65472-v • 05:34 min • July 14th, 2023
July 14th, 2023 •
1, 1,2, 1, 1
1Unità di Genetica, Dipartimento di Biologia, University of Pisa, 2Department of Women-Child-Newborn Obstetrics and Gynaecology, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico

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This protocol presents a method to assess the formation and repair of DNA double-strand breaks through the simultaneous detection of γH2AX and 53BP1 foci in interphase nuclei of bleomycin-treated human peripheral lymphocytes.

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Dual Immunofluorescence &946;H2AX 53BP1 Human Peripheral Lymphocytes Genomic Damage Bleomycin Double Strand Breaks Repair Mechanisms Co-localization Whole Blood Samples Complete Medium Negative Control Thermostat Centrifuge Hypotonic Solution Pre Fixative Solution Hemolysis Methanol Fixation Fixative Solution Slides
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