Идентификация связывающих белков малых лигандов с дифференциальным радиальным капиллярным действием лигандного анализа (DRaCALA)

0:42

Preparation of Whole Cell Lysates

1:43

Nickel-Nitrilotriacetic Acid (Ni-NTA) Affinity Purification of Relseq and GppA

2:55

Gel Filtration

3:33

Synthesis of ³²P-Labeled Guanosine Pentaphosphate (pppGpp) and Guanosine Tetraphosphate (ppGpp)

5:08

DRaCALA Screening of the Target Proteins of (p)ppGpp

6:37

Quantification and Identification of Potential Target Proteins

7:46

Results: DRaCALA Plate Screening and Quantification

8:39

Conclusion