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Capítulo 11
Atenuação da Transcrição em Procariontes
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin…
Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins.…
Edição de RNA
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or…
Transporte Regulado de mRNA
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to…
Varredura de Vazamento
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start…
Estabilidade de mRNA e Expressão Gênica
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes,…
Interferência do RNA
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the expression of a gene by binding to its messenger RNA (mRNA)…
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of…
siRNA - Pequenos RNAs de Interferência
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional…
piRNA - RNAs que interagem com Piwi
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs…
CRISPR e crRNAs
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to…
lncRNA - RNAs Não Codificantes Longos
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces…
Ribozimas
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower…
Condições na Terra Primitiva
Around 4 billion years ago, oceans began to condense on earth while volcanic eruptions released nitrogen, carbon dioxide, methane, ammonia, and…
Mesclando Absoluto e dados relativos PCR quantitativo para quantificar STAT3 Splice Variant Transcrições
Human signal transducer and activator of transcription 3 (STAT3) is one of many genes containing a tandem splicing site. Alternative donor splice…
Sistema de expressão plasmídeamente controlado transcricionalmente para investigação da estabilidade das transcrições de mRNA em células epiteliais alveolares primárias
Studying posttranscriptional regulation is fundamental to understanding the modulation of a given messenger RNA (mRNA) and its impact on cell…
Interferência de RNA em besouros aquáticos como uma poderosa ferramenta para manipular a expressão genética em pontos de tempo específicos de desenvolvimento
RNA interference (RNAi) remains a powerful technique that allows for the targeted reduction of gene expression through mRNA degradation. This…

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