SC1 functions through dual inhibition of Ras- GAP and ERK1. We tested the function of SC1 in supporting mouse ES cell self-renewal in the absence of LIF and showed that SC1 is able to maintain self-renewal of mouse ES cell cultures.
The small molecule SC1 can be used to maintain self-renewal with an undifferentiated state in mouse ES cells. Before using on an untested cell line, however, it should be titrated to determine the optimal concentration. For example, we tested three concentrations on the mouse ES cell line ESC R1. 100 nM and 300 nM concentrations were able to sustain mouse ES cells, however, a 1 μM concentration was toxic.
SC1 can also be used under feeder-free conditions.
The authors have nothing to disclose.
The authors wish to thank Dr. Sheng Ding of The Scripps Research Institute and Dr. Hongkui Deng of Peking University for their assistance and direction.
Material Name | Type | Company | Catalogue Number | Comment |
---|---|---|---|---|
SC1 | Stemgent | 04-0011 | ||
LIF | Millipore | ESG1107 | ||
SSEA-1 antibody | Santa Cruz | 21702 | ||
Nanog antibody | Abcam | ab21603 | ||
Sox2 antibody | Chemicon | Ab5603 | ||
Oct4 antibody | Santa Cruz | SC-5279 |