Intrabronchial Delivery: A Technique to Administer an Experimental Agent Selectively Into a Mouse Lung

Abstract

Source: Liao, S. et. al. Direct Intrabronchial Administration to Improve the Selective Agent Deposition Within the Mouse Lung. J. Vis. Exp. (2019).

This video describes an intrabronchial approach to administer an experimental agent selectively into distal right and left lung. In the featured protocol, we will administer bleomycin intrabronchially into a mouse to obtain a better model for pulmonary fibrosis.

Protocol

All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Non-operative IB intubation and delivery approaches

1. IB approach to selective lobar cannulation of the distal right lung
   1. After performing intratracheal (IT) cannulation (use the dominant hand to intubate the extended catheter into the trachea via the oral cavity), rotate the plastic board +30° (Figure 1A).
   2. Hold the hub of the catheter and guide it naturally in parallel to the mouse midline, extending it to weight-based d....

Results

Figure 1: Approach for selective right/left lung lobar cannulation and administration. (A) To target the right lung, the plastic board is rotated +30°, improving ease of selectively engaging the rig.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
22 G shielded IV Catheter 	 BD	381423
Evans blue dye 	Sigma-Aldrich	 E2129
LED-30W Fiber Optic Dual Gooseneck Lights Microscope Illuminator 	AmScope	 LED-30W
Precisionglide needle, 18G x 1"  	 BD	305195	Beveled tip, 12 mm in length