Formalin-Fixed Paraffin Embedded (FFPE) Tissue Preservation: A Method for Studying Tissue Morphology

In This Article

Abstract

Source: Nagaraj A.S., et al., Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing. J. Vis. Exp. (2018)

The following video describes how to preserve morphology of a tissue with formalin fixation and paraffin embedding.

Protocol

1. Fixation and Processing of the Tissue Slices

Carefully lift the uncultured 0 h reference or cultured slice onto a filter paper soaked in PBS.
1. To do this, add 2-3 mL of PBS on top of a filter paper placed in a 10 cm plate. Place the slice on top of the filter paper and lift out the filter paper using a pair of forceps.
2. Transfer the filter paper into a histocassette, and add a drop of diluted hematoxylin (1:1 in deionized water) on top of the tissue slice to visibly mark the position of the slice during the subsequent processing steps (Figure 1D).
3. Close the cassette, and transfer it into 4% neutral buffer....

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Results

Lung tumor sectioning for analysis: dissection, vibratome slicing, cultivation, FFPE block generation.
Figure 1: Schematic representation of the workflow for establishment and analysis of murine NSCLC tumor-derived slice explants. (A) Schematic describing the collection and preparation of tumor-bearing lungs for slicing. Lung lobes are.......