Title Cell Encapsulation by Droplets

Method Article

DOI:

⸱

October 1st, 2007

Protocol

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NIH3T3 cells preparation:

A. Cells for Ejection

Trypsinize cells, then dilute 1:1 with cell media, and transfer from a T75 flask to a 15 mL Falcon tube
Spin down cells into a pellet by centrifuging, aspirate supernatant and wash cells with DPBS
Spin down cells into a pellet again, and aspirate supernatant
Resuspend cells in media
Determine cell density with hemocytometer (~200 X 10⁴ cells/mL per T75 flask)
Centrifuge cell solution, aspirate supernatant, and resuspend in appropriate amount of media for varying cell concentrations

B. Cell ejection<....

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The authors have nothing to disclose.

List of materials used in this article
Name	Company	Catalog Number	Comments
Fluorescent Microscope		Nikon Instruments	Eclipse TE-2000 U
Solenoid valve cell ejector				Operation frequency: 1 KHz, 30psi, 50nl~0.5ul. 12V Valve Driver: 2.5 Amp drive current
5-Gallon Portable air tank		Coleman	Powermate CT5	Pressurized air: 30 PSI
Pressure regulators		Marsh Bellofram
Pulse Generator			HP8112A	Actuation frequency generation: 50 MHz
XYZ-stage		Newmark Systems		With Stepping motor: 6inch travel xy-stage(NLS4-6-25), 2.5inch travel z-stage(NLS4-2.5-25), 3axis controller(NSC-G3), 0.1um resolution
NIH 3T3	Cell-line			fibroblasts
Trypsin				0.05% solution
NIH 3T3 cell medium
DPBS	Buffer
T75				Tissue culture flasks
Plastic conical tubes				15 ml, for tissue culture

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