Materials
| Name | Company | Catalog Number | Comments |
| Name of the reagent | Company | Catalogue number | Comments (optional) |
| 535 nm Laser | Crylas | FDSS532Q3 | (TEEM, Crystal, and CRC also offer comparable lasers) |
| All optics and hardware | (Thor offers comparable optics and hardware) | ||
| SUPREMA Optical Mount, 1.0-in diameter | Newport Corp. | SS100-F2KN | 2 |
| 1" diameter post, 1" height | Newport Corp. | PS-1 | 1 |
| 1" diameter post fork | Newport Corp. | PS-F | 1 |
| Achromatic Zero-Order Wavel Plate, ½ Wave Retardation, 400-700nm | Newport Corp. | 10RP52-1 | 1 |
| Rotation Stage, 1" Aperature | Newport Corp. | RSP-1T | 1 |
| 1" diameter post, 1" height | Newport Corp. | PS-1 | 1 |
| 1" diameter post fork | Newport Corp. | PS-F | 1 |
| Glan-Laser Calcite Polarizer, 430-700nm | Newport Corp. | 10GL08AR.14 | 1 |
| Polarizer Rotation Mount | Newport Corp. | RM25A | 1 |
| ¼" spacer for 1" diameter post | Newport Corp. | PS-0.25 | 1 |
| ½" height, 1"diameter post | Newport Corp. | PS-0.5 | 1 |
| Fork, 1" diameter post | Newport Corp. | PS-F | 1 |
| Beam Dump | Newport Corp. | PL15 | 1 |
| Microscope Objective Lens Mount | Newport Corp. | LH-OBJ1 | 1 |
| 1" diameter post, 1" height | Newport Corp. | PS-1 | 1 |
| 1" diameter post fork | Newport Corp. | PS-F | 1 |
| High-Energy Nd:YAG Laser Mirror, 25.4 mm Diameter, 45°, 532 nm | Newport Corp. | 10Q20HE.2 | 4 |
| SUPREMA Optical Mount, 1.0 inch diameter, clearance mounting hole | Newport Corp. | SN100C-F | 2 |
| High Precision Knob Adjustment Screw, 12.7mm travel, 100TPI | Newport Corp. | AJS100-0.5K | 4 |
| Thread adaptor, ¼-20 male, 8-32 female | Newport Corp. | SS-1-B | 2 |
| Rod Clamp for 1.5 inch diameter rod | Newport Corp. | 340-RC | 2 |
| Rod, 14 inch (35.5 cm) tall | Newport Corp. | 40 | 1 |
| Rail carrier for X26, square 40mm length | Newport Corp. | CN26-40 | 4 |
| Steel Rail, 384mm (15") length | Newport Corp. | X26-384 | 1 |
| Rod Platform for 1.5 inch diameter rod | Newport Corp. | 300-P | 2 |
| Rod, 14 inch (35.5 cm) tall | Newport Corp. | 40 | 2 |
| Plano-Concave Lens, 12.7mm diameter, -25mm EFL, 430-700nm | Newport Corp. | KPC025AR.14 | 2 |
| Plano-Convex Lens, 25.4mm diameter, 50.2mm EFL, 430-700nm | Newport Corp. | KPX082AR.14 | 1 |
| Plano-Convex Lens, 25.4mm diameter, 62.9mm EFL, 430-700nm | Newport Corp. | KPX085AR.14 | 1 |
| Fixed Lens Mount, 0.5" diameter, 1.0" Axis height | Newport Corp. | LH-0.5 | 2 |
| Fixed Lens Mount, 1.0" diameter, 1.0" Axis height | Newport Corp. | LH-1 | 2 |
| Microspheres 0.1um | Polysciences, Inc. | 00876 | |
| Agarose | Research Products International Corp. | A20090 | EEO matters |
| Muscimol | Sigma-Aldrich | M1523 |
References
- Hammarlund, M., Nix, P., Hauth, L., Jorgensen, E. M., Bastiani, M. Axon regeneration requires a conserved MAP kinase pathway. Science. 323, 802-806 (2009).
- Steinmeyer, J. D. Construction of a femtosecond laser microsurgery system. Nature protocols. 5, 395-407 (2010).
- Wu, Z. Caenorhabditis elegans neuronal regeneration is influenced by life stage, ephrin signaling, and synaptic branching. Proc Natl Acad. 104, 15132-15137 (2007).
- Gilleland, C. L., Rohde, C. B., Zeng, F., Yanik, M. F. Microfluidic immobilization of physiologically active Caenorhabditis elegans. Nature protocols. 5, 1888-1902 (2010).
- Raabe, I., Vogel, S. K., Peychl, J., Tolic-Norrelykke, I. M. Intracellular nanosurgery and cell enucleation using a picosecond laser. J. Microsc. 234, 1-8 (2009).
- Hutson, M. S., Ma, X. Plasma and cavitation dynamics during pulsed laser microsurgery in vivo. Physical review letters. 99, 158104-158104 (2007).
- Venugopalan, V., Guerra, A. 3rd, Nahen, K., Vogel, A. Role of laser-induced plasma formation in pulsed cellular microsurgery and micromanipulation. Physical review letters. 88, 078103-078103 (2002).
- Bourgeois, F., Ben-Yakar, A. Femtosecond laser nanoaxotomy properties and their effect on axonal recovery in C. elegans. Opt Express. 16, 5963-5963 (2008).
- O'Brien, G. S., Rieger, S., Martin, S. M., Cavanaugh, A. M., Portera-Cailliau, C., Sagasti, A. Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos. J. Vis. Exp. (24), e1129-e1129 (2009).
- Tsai, P. S. Plasma-mediated ablation: an optical tool for submicrometer surgery on neuronal and vascular systems. Curr. Opin. Biotechnol. 20, 90-99 (2009).
- Chung, S. H., Clark, D. A., Gabel, C. V., Mazur, E., Samuel, A. D. The role of the AFD neuron in C. elegans thermotaxis analyzed using femtosecond laser ablation. BMC Neurosci. 7, 30-30 (2006).
- Shen, N. Ablation of cytoskeletal filaments and mitochondria in live cells using a femtosecond laser nanoscissor. Mech. Chem. Biosyst. 2, 17-25 (2005).
- Rao, G. N., Kulkarni, S. S., Koushika, S. P., Rau, K. R. In vivo nanosecond laser axotomy: cavitation dynamics and vesicle transport. Opt. Express. 16, 9884-9894 (2008).
- Rohde, C. B., Yanik, M. F. Subcellular in vivo time-lapse imaging and optical manipulation of Caenorhabditis elegans in standard multiwell plates. Nat. Commun. 2, 271-271 (2011).
