Materials
| Name | Company | Catalog Number | Comments |
| Name of the reagent/equipment | Company | Catalogue number | Comments |
| Air tank and regulator | Airgas Mid America | UN3156 | For pressurizing myograph |
| Pressure myograph and software | Danish Myotechnology | 110P, MyoView | With custom cannulae (Figure 2) |
| Inverted microscope, 5x lens and camera | Zeiss | Axiovert 40C | For tracking artery diameter |
| Physiological saline solution (PSS) | Chemicals from Sigma | Recipe and details in Table 2 | |
| Surgical tape | Various suppliers | For securing the mouse during dissection | |
| Dissection board | Fisher Scientific | 09-002-24A | For securing mouse during dissection |
| Dissecting microscope with camera | Zeiss | Stemi 2000-C |
For arterial dissection and mounting |
| Dissecting scissors | Fine Science Tools | 14058-11 | For cutting skin and opening the chest |
| Fine tweezers (2) | Fine Science Tools | 11200-14 | For grasping artery ends |
| Curved forceps | Fine Science Tools | 11274-20 | For clearing tissue and exposing carotid arteries |
| Micro-scissors | Fine Science Tools | 15005-08 | For precise cutting of arteries |
| 7-0 and 10-0 silk suture | Various suppliers | For estimating length and fastening arteries on cannulae | |
| Digital calipers | Fisher Scientific | 806-93-111 | For measuring suture length and checking artery length |
| Disposable scalpel | Feather | No. 15 | For cutting artery rings |
| Activated charcoal | Sigma | C4386-500G | For marking cut locations on vessels |
| 18G Needle | Beckton-Dickinson | 305136 | For applying activated charcoal to vessels, clearing blood and filling myograph tubing |
| 20 mL syringe | Various suppliers | For clearing blood and filling myograph tubing | |
| Petri dish | Fisher Scientific | 08-757-13B | For inserting vessels after dissection and testing to take pictures |
| Microfuge tube | Fisher Scientific | 02-682-550 | For storing vessels before testing |
| Fine wire | California Fine Wire Company | 100192 | For clearing clogged cannula |
| ImageJ software | National Health Institute | www. rsbweb.nih.gov/ij | Open-source image processing program developed by NIH |
| Matlab software | Mathworks | Useful for analyzing data and fitting constitutive equations |
References
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