Terahertz Microfluidic Sensing Using a Parallel-plate Waveguide Sensor

Summary

The procedure for implementing a refractive index sensor for terahertz frequencies based on a grooved parallel-plate waveguide geometry is described here. The method yields a measurement of the refractive index of a small volume of liquid through monitoring of the shift in the resonant frequency of the waveguide structure

Abstract

Refractive index (RI) sensing is a powerful noninvasive and label-free sensing technique for the identification, detection and monitoring of microfluidic samples with a wide range of possible sensor designs such as interferometers and resonators ^1,2. Most of the existing RI sensing applications focus on biological materials in aqueous solutions in visible and IR frequencies, such as DNA hybridization and genome sequencing. At terahertz frequencies, applications include quality control, monitoring of industrial processes and sensing and detection applications involving nonpolar materials.

Several potential designs for refractive index sensors in the terahertz regime exist, including photonic crystal waveguides ³, asymmetric split-ring resonators ⁴, and photonic band gap structures integrated into parallel-plate waveguides ⁵. Many of these designs are based on optical resonators such as rings or cavities. The resonant frequencies of these structures are dependent on the refractive index of the material in or around the resonator. By monitoring the shifts in resonant frequency the refractive index of a sample can be accurately measured and this in turn can be used to identify a material, monitor contamination or dilution, etc.

The sensor design we use here is based on a simple parallel-plate waveguide ^6,7. A rectangular groove machined into one face acts as a resonant cavity (Figures 1 and 2). When terahertz radiation is coupled into the waveguide and propagates in the lowest-order transverse-electric (TE₁) mode, the result is a single strong resonant feature with a tunable resonant frequency that is dependent on the geometry of the groove ^6,8. This groove can be filled with nonpolar liquid microfluidic samples which cause a shift in the observed resonant frequency that depends on the amount of liquid in the groove and its refractive index ⁹.

Our technique has an advantage over other terahertz techniques in its simplicity, both in fabrication and implementation, since the procedure can be accomplished with standard laboratory equipment without the need for a clean room or any special fabrication or experimental techniques. It can also be easily expanded to multichannel operation by the incorporation of multiple grooves ¹⁰. In this video we will describe our complete experimental procedure, from the design of the sensor to the data analysis and determination of the sample refractive index.

Protocol

1. Sensor Design and Fabrication

1. Design a parallel-plate waveguide with one or more integrated cavities (or "grooves"). See Figures 1 and 2. Geometry may be based on that given in our previous publications ^8,9 or specially designed for the particular application. The following general guiding principles are suggested:
   1. Plate Spacing: In this experiment a plate spacing of 1mm is used for effective coupling to the TE1 mode without the need for special optics. It also ensures single-mode propagation at the frequencies of interest. When using other plate spacings, multimode propagation, dispersion and cou....

Discussion

It should be noted that the refractive index of the liquid under test is determined only at the frequency of the cavity resonance, not over a broad bandwidth. This has a few distinct advantages. First, although our measurements have made use of a broadband terahertz source for characterization purposes, one could also build an equivalent sensing system with a single-frequency THz source with only a limited degree of frequency tunability, an approach that could be much less expensive and more compact. Second, the .......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
10 μl syringe	Hamilton	80314	High precision syringe
Liquid alkanes	Acros Organics		Samples for calibration and testing
No specific equipment is required. Suitable test materials and solvents are left to the experimenter's discretion. The high-precision syringes used in this procedure are listed in the table below, but the experimenter may wish to use syringes of a different volume or design, including digital syringes for improved accuracy. The test alkanes used in this experiment are also listed.