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Neuroscience

Двойной Tracer ПЭТ-МРТ протокол для количественного измерения регионального мозга энергетических субстратов Поглощение в Крысы

Published: December 28, 2013 doi: 10.3791/50761
Automatic Translation
1, 1, 2, 3, 2, 2, 2,4, 1
1Research Center on Aging and Department of Physiology and Biophysics, Université de Sherbrooke, 2Sherbrooke Molecular Imaging Center, Étienne-Le Bel Clinical Research Center, Université de Sherbrooke, 3Department of Computer Science, Université de Sherbrooke, 4Department of Nuclear Medicine and Radiobiology, Université de Sherbrooke

Materials

Name Company Catalog Number Comments
MRI scanner Varian 7 Tesla
Small-animal PET scanner Gamma Medica Lab-PET/-Triumph
Heat mat Sunbeam PN 143937
Heater system SA Instruments 761 100 Rev B
Respiratory gating SA Instruments SAII's P-resp
Clinical chemistry analyzer Siemens Healthcare Diagnosis 765000.931 Dimension Xpand Plus
Polyethylene tubing 50  Becton Dickinson 427411
Injection pump KD Scientific Model 210
Gamma-counter GMI Packard Cobra II
Centrifuge Thermo Scientific 75002416 Heraeus Pico 21
PMOD software PMOD Technologies PMOD 3.2 version
Geiger counter Fluke Biomedical ASM-990 Advanced Survey Meter
Reagent
Isoflurane Abbott Laboratories, Ltd B506
0.9% NaCl solution Hospira 4888010
Heparin Sandoz 1004336
Isopropenyl acetate Aldrich 11778 99%
Methyllithium Aldrich 197343 1.6 M
THF Aldrich 87371
Flex reagent cartridge glucose Siemens Healthcare Diagnosis DF40
Trizma base Sigma T6066-500G prepare tris buffer 100 mM pH 7.0
Sodium oxamate Sigma O2751 20 mM
NADH Roche 10128015001 0.15 mM
b-Hydroxybutyrate dehydrogenase Toyobo HBD-301 1 U/ml

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References

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  16. Croteau, E., et al. Image-derived input function in dynamic human PET/CT: methodology and validation with 11C-acetate and 18F-fluorothioheptadecanoic acid in muscle and 18F-fluorodeoxyglucose in brain. Eur. J. Nucl. Med. Mol. Imaging. 37, 1539-1550 (2010).
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Двойной Tracer ПЭТ-МРТ протокол для количественного измерения регионального мозга энергетических субстратов Поглощение в Крысы
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Cite this Article

Roy, M., Nugent, S., Tremblay, S.,… More

Roy, M., Nugent, S., Tremblay, S., Descoteaux, M., Beaudoin, J. F., Tremblay, L., Lecomte, R., Cunnane, S. C. A Dual Tracer PET-MRI Protocol for the Quantitative Measure of Regional Brain Energy Substrates Uptake in the Rat. J. Vis. Exp. (82), e50761, doi:10.3791/50761 (2013).

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