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Bioengineering

Human primat Lung Decellularization og Recellularization bruge en specialiseret Large-orgel bioreaktor

doi: 10.3791/50825 Published: December 15, 2013

Materials

Name Company Catalog Number Comments
Sodium nitroprusside Sigma-Aldrich 71778-25G
Heparin sodium salt Fisher BP2425
Triton X-100 Fisher BP151-100
Sodium deoxycholate Fisher BP349-100
Sodium chloride Fisher 7647-14-5
Bovine pancreatic DNase Sigma-Aldrich DN25 Prepare stock, aliquot, and freeze
Magnesium sulfate Fisher 10034-99-8
Calcium chloride Fisher C614-500
PBS (no Ca/Mg) Gibco, Life Technologies 10010-031
Antibiotic-Antimycotic Gibco Life Technologies 15240062
Cell Culture Media
Alpha MEM Gibco Life Technologies 12561-072
Medium 199 Gibco Life Technologies 11150067
Premium Fetal Bovine Serum Atlanta Biologicals S11150
L-Glutamine 100x Gibco Life Technologies 25030-081
Endothelial Cell Growth Supplement (ECGS) ScienCell 1052
Antibiotic-Antimycotic Gibco Life Technologies 15240062
Cell Seeding and Bioreactor Culture
Check valves Cole-Parmer EW-98553-20
Y-connectors Cole-Parmer ED-30614-08
3-Way stopcocks Harvard Apparatus 721664
MasterFlex L/S 14 tubing Cole-Parmer 96420-14
MasterFlex L/S 16 tubing Cole-Parmer 96420-16
Male lock Luer 1/8 in Cole-Parmer EW-45505-04
Female Luer 1/8 in Cole-Parmer SI-45502-04
Male Luer lock plug Cole-Parmer EW-45505-56
Injection ports Medi-Dose EPS IV2004
Latex tubing St. Louis Medical Suppy HN10910
Hose clamp Cole-Parmer EW-06832-02
2 L Wide-mouth jar Fisher 05-719-276
60 ml Syringes Fisher NC9035364

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References

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  2. Badylak, S. F., Taylor, D., Uygun, K. Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds. Annu. Rev. Biomed. Eng. 13, 27-53 (2011).
  3. Ott, H. C., et al. Regeneration and orthotopic transplantation of a bioartificial lung. Nat. Med. 16, 927-933 (2010).
  4. Price, A. P., England, K. A., Matson, A. M., Blazar, B. R., Panoskaltsis-Mortari, A. Development of a decellularized lung bioreactor system for bioengineering the lung: the matrix reloaded. Tissue Eng. Part A. 16, 2581-2591 (2010).
  5. Petersen, T. H., et al. Tissue-engineered lungs for in vivo implantation. Science. 329, 538-541 (2010).
  6. Song, J. J., et al. Enhanced in vivo function of bioartificial lungs in rats. Ann. Thorac. Surg. 92, 998-1005 (2011).
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  11. Fabbrini, E., et al. Metabolic response to high-carbohydrate and low-carbohydrate meals in a non-human primate model. Am. J. Physiol. Endocrinol. Metab. (2012).
  12. Crapo, P. M., Gilbert, T. W., Badylak, S. F. An overview of tissue and whole organ decellularization processes. Biomaterials. 32, 3233-3243 (2011).
  13. Calle, E. A., Petersen, T. H., Niklason, L. E. Procedure for Lung Engineering. J. Vis. Exp. (49), e2651 (2011).
  14. Arold, S. P., Wong, J. Y., Suki, B. Design of a new stretching apparatus and the effects of cyclic strain and substratum on mouse lung epithelial-12 cells. Ann. Biomed. Eng. 35, 1156-1164 (2007).
  15. Huang, H., et al. Differentiation from embryonic stem cells to vascular wall cells under in vitro pulsatile flow loading. J. Artif. Organs. 8, 110-118 (2005).
  16. Izadpanah, R., et al. Characterization of multipotent mesenchymal stem cells from the bone marrow of rhesus macaques. Stem Cells Dev. 14, 440-451 (2005).
  17. Craig, L. E., Nealen, M. L., Strandberg, J. D., Zink, M. C. Differential replication of ovine lentivirus in endothelial cells cultured from different tissues. Virology. 238, 316-326 (1006).
  18. Craig, L. E., Spelman, J. P., Strandberg, J. D., Zink, M. C. Endothelial cells from diverse tissues exhibit differences in growth and morphology. Microvasc. Res. 55, 65-76 (1998).
  19. Bonvillain, R. W., et al. A Nonhuman Primate Model of Lung Regeneration: Detergent-Mediated Decellularization and Initial In Vitro Recellularization with Mesenchymal Stem Cells. Tissue Eng. Part A. 18, 2437-2452 (2012).
  20. Badylak, S. F., Taylor, D., Uygun, K. Whole-Organ Tissue Engineering: Decellularization and Recellularization of Three-Dimensional Matrix Scaffolds. Annu. Rev. Biomed. Eng. (2010).
Human primat Lung Decellularization og Recellularization bruge en specialiseret Large-orgel bioreaktor
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Bonvillain, R. W., Scarritt, M. E., Pashos, N. C., Mayeux, J. P., Meshberger, C. L., Betancourt, A. M., Sullivan, D. E., Bunnell, B. A. Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor. J. Vis. Exp. (82), e50825, doi:10.3791/50825 (2013).More

Bonvillain, R. W., Scarritt, M. E., Pashos, N. C., Mayeux, J. P., Meshberger, C. L., Betancourt, A. M., Sullivan, D. E., Bunnell, B. A. Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor. J. Vis. Exp. (82), e50825, doi:10.3791/50825 (2013).

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