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Medicine

쥐의 대동맥 부비동의 동맥 경화 병변의 정량 분석​​ 및 특성

Published: December 7, 2013 doi: 10.3791/50933

Materials

Name Company Catalog Number Comments
LDLR-deficient mice (D2.129S7(B6)-Ldlrtm1Her/J) The Jackson Laboratory stock #002207
Formalin Sigma-Aldrich HT5011
Histology Molds Ted Pella Inc 27195
Paraffin Paramat 19286-10
Coated slides Fisher 12-550-15
Solvent containers Tissue Tek II 4457
Microtome Leica RM22S5
Water bath VWR 80086-982
Microscope Olympus BX41TF
Camera Olympus DP72
ImageJ NIH www.rsbweb.nih.gov/ij/
Processor Sakura Finetek VIP6-A1
Processing cassettes VWR 18000-134
Preassure Cooker Nordic Ware
Coverslip Fisher 12-545-M
PBS Sigma-Aldrich P3813
Microtome blade Thermo Scientific 30-518-35
Antifade mounting medium Sigma-Aldrich F-4680
Xylene mounting medium  Sigma-Aldrich 44581
Aquous mounting medium Sigma-Aldrich I1161
Mac-3 anbody BD Pharmingen 553322
DAPI Sigma-Aldrich D9542
F4/80 antibody Abcam Ab6640
Alpha actin antibody Santa Cruz Biotechnology SC-32251
Mayer’s Hematoxylin Sigma-Aldrich E4382
Eosin Y Sigma-Aldrich MHS16
DAB (3,3'-Diaminobenzidine) Dako K3468

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References

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  17. Lee, T. Y., Muschal, S., et al. Angiostatin regulates the expression of antiangiogenic and proapoptotic pathways via targeted inhibition of mitochondrial proteins. Blood. 114 (9), 1987-1998 (2009).
  18. Ballon, G., Chen, K., et al. Kaposi sarcoma herpesvirus (KSHV) vFLIP oncoprotein induces B cell transdifferentiation and tumorigenesis in mice. J. Clin. Invest. 121 (3), 1141-1153 (2011).
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  21. Kanellakis, P., Agrotis, A., et al. High-mobility group box protein 1 neutralization reduces development of diet-induced atherosclerosis in apolipoprotein e-deficient mice. Arterioscler. Thromb. Vasc. Biol. 31 (2), 313-319 (2011).
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  27. Kirkby, N. S., Low, L., et al. Quantitative 3-dimensional imaging of murine neointimal and atherosclerotic lesions by optical projection tomography. PLoS One. 6 (2), e16906 (2011).
쥐의 대동맥 부비동의 동맥 경화 병변의 정량 분석​​ 및 특성
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Cite this Article

Venegas-Pino, D. E., Banko, N.,More

Venegas-Pino, D. E., Banko, N., Khan, M. I., Shi, Y., Werstuck, G. H. Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus. J. Vis. Exp. (82), e50933, doi:10.3791/50933 (2013).

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