Materials
| Name | Company | Catalog Number | Comments |
| Fixing | |||
| Alcohol-Formalin-Acetic Acid (AFA) | Fisher Scientific | A407-1 (ethanol) F75F-1GAL (formaldehyde) BP2401500 (glacial acetic acid) |
Ethanol 85% (85 ml) + formaldehyde 37% (10 ml) + glacial acetic acid (5 ml) |
| Ethanol for killing and long-term preservation | Fisher Scientific | A407-1 | Ethanol 100% (70 ml) + distilled water (30 ml) |
| Ethanol for fixing and DNA studies | Fisher Scientific | AC61511-0010 | Ethanol 80-90% (80-90 ml) + distilled water (20-10 ml), respectively |
| Formalin Buffered | Fisher Scientific | SF100-4 | Formalin 10% (10 ml) + distilled water (90 ml) |
| Glycerine-alcohol | Fisher Scientific | A407-1 (ethanol) AC15365-1000 (glycerine) |
Ethanol 70% (95 ml) + glycerine (5 ml) |
| Clearing, staining and mounting | |||
| Ethanol for dehydrating during staining | Fisher Scientific | A407-1 | Ethanol 80%, 95%, 100% (80 ml, 95 ml, 100 ml) + distilled water (20 ml, 5 ml, 0 ml) |
| Lactophenol | Fisher Scientific | R400-27 | - |
| Phenol | Fisher Scientific | A931I-1 (phenol) A407-1 (ethanol) |
Phenol 100% (80 ml) + ethanol 100% (20 ml) |
| Harris’ Hematoxylin | Fisher Scientific | S25347 (hematoxylin) A407-1 (ethanol) A567-500 (ammonium aluminum sulfate) S25553 (sodium iodate) |
Hematoxylin (5 g) + ethanol 100% (50 ml) + ammonium aluminum sulfate (100 g) + sodium iodate (0.37 g) + distilled water (1,000 ml) |
| Semichon’s acetic carmine | Fisher Scientific | BP2401500 (glacial acetic acid) S25236 (carmine) A407-1 (ethanol) |
Glacial acetic acid (100 ml) + carmine (1.5 g) + distilled water (100 ml). Heat in a boiling water bath for 15 min and cool. Filter to make stock solution. Mix 1:2 with 70% alcohol for staining. |
| Xylene | Fisher Scientific | X4-4 | Use only under a chemical fume hood. |
| Methyl salicylate | Fisher Scientific | S25437 | - |
| Ethanol basic | Fisher Scientific | A407-1 (ethanol) S25159A (concentrated ammonium hydroxide) |
Ethanol 70% (100 ml) + ammonium hydroxide (0.1 ml) |
| Ethanol acid | Fisher Scientific | A407-1 (ethanol) SA9233-100 (hydrochloric acid) |
Ethanol 70% (99.5 ml) + hydrochloric acid (0.5 ml) |
| Canada balsam | Fisher Scientific | B10-100 | - |
References
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