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Medicine

Intravital Video Mikroskopi Målinger af Retinal blodgennemstrømningen i Mus

Published: December 26, 2013 doi: 10.3791/51110
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1, 1, 1
1Department of Molecular & Cellular Physiology, Louisiana State University Health Sciences Center

Materials

Name Company Catalog Number Comments
Fluorescent microspheres Bangs Laboratories FS04F/10584 (green)
High molecular weight fluorescent dextran Molecular Probes D-7137 (green); D-7139 (red)
Microscope system Nikon Eclipse E600FN + attachments
4X objective Nikon Plan Fluor 4X numerical aperture 0.13; working distance 17.2 mm
10X objective Nikon Plan 10X numerical aperture 0.25; working distance 10.5 mm
Tropicamide ophthalmic solution Bausch Lomb 1% Tropicamide
Hypromellose ophthalmic solution HUB Pharmaceuticals 2.5% Goniovisc
Image processing software University of California San Francisco Vale Lab Micro-Manager
Digital video camera for microscopy Photometrics CoolSnap ES 1392 x 1040 pixel resolution; pixel size 6.45 x 6.45 μm

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References

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Intravital Video Mikroskopi Målinger af Retinal blodgennemstrømningen i Mus
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Harris, N. R., Watts, M. N.,… More

Harris, N. R., Watts, M. N., Leskova, W. Intravital Video Microscopy Measurements of Retinal Blood Flow in Mice. J. Vis. Exp. (82), e51110, doi:10.3791/51110 (2013).

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