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طرق لتسهيل النمو الميكروبي على اللب مطحنة النفايات تيارات وتوصيف إمكانات التحلل الحيوي من الميكروبات مثقف

doi: 10.3791/51373 Published: December 12, 2013

Materials

Name Company Catalog Number Comments
1,4-Dioxane (Certified ACS) Fisher D111 Flammable, preoxidizable chemical
Black Liquor Department of Forest Biomaterials at North Carolina State University N/A pH 12.72, 13.67% solids
Ethanol (microbiology grade) Fisher BP2818
Ethyl Acetate (ACS reagent grade) EMD EX0240-9 Flammable
Glacial acetic acid (Certified ACS) EMD AX0073 Corrosive
Guaiacol Sigma Aldrich PHR1136 Harmful by ingestion, corrosive
HP-5 capillary column Agilent 19091J-577 60 m x 0.18 mm internal diameter, 0.18 μm thickness
Hydrochloric acid (certified ACS) EMD HX0603P
N,O-Bis(trimethylsilyl)trifluoroacetamide with trimethylcholorsilane 99:1% (BSTFA) Fluka 15238 Flammable, causes skin burns and eye damage with contact
Na2SO4 (FCC grade) VWR BDH8026
NaClO2 (80%) Sigma Aldrich 244155 Flammable, toxic  
NaOH (certified ACS) EMD 1.06498.1000 Corrosive
Alkacid pH test ribbons Fisher A979
Phosphoric Acid (certified ACS) Fisher A242
Polaris Q mass spectrometer  Thermo Electron Corporation
Pyridine (99%) Alfa Aesar A12005 Flammable, toxic in contact with skin
Switchgrass Cherry Research Farm Goldsboro, NC N/A Harvested August 2011
Thermo Finnigan trace gas chromatograph Thermo Electron Corporation
Whatman no. 1 filter paper Whatman 1001-150

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References

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  13. Lech, K., Brent, R. Short protocols in molecular biology. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., Kevin, S. 2nd, John Wiley & Sons. (1992).
  14. Raj, A., Krishna Reddy, M. M., Chandra, R. Identification of low molecular weight aromatic compounds by gas chromatography-mass spectrometry (GC-MS) from kraft lignin degradation by three Bacillus sp. Int. Biodeterior. Biodegradation. 59, 292-296 (2007).
  15. Chandra, R., Raj, A., Purohit, H. J., Kapley, A. Biodegradation of kraft lignin by three pure and mixed aerobic bacterial cultures isolated from pulp and paper sludge. Proceeding of the 25th international symposium on toxicology environmental and occupational health, (2005).
  16. Chen, Y., Chai, L., Tang, C., Yang, Z., Zheng, Y., Shi, Y., Zhang, H. Kraft lignin biodegradation by Novosphingobium sp. B-7 and analysis of the degradation process. Bioresource Technol. 123, 682-685 (2012).
  17. Bandounas, L., Wierckx, N. J., de Winde, J. H., Ruijssenaars, H. J. Isolation and characterization of novel bacterial strains exhibiting ligninolytic potential. BMC Biotechnol. 11, (94), (2011).
  18. Chandra, R., Abhishek, A., Sankhwar, M. Bacterial decolorization and detoxification of black liquor from rayon grade pulp manufacturing industry and detection of their metabolic products. Bioresource Technol. 102, 6429-6436 (2011).
  19. Gellerdtedt, G. Chemistry of Bleaching of Chemical Pulp. Pulp and Paper Chemistry and Technology. Ek, M., Gellerstedt, G., Henriksson, G. 2, De Gruyter. 201-237 (2009).
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طرق لتسهيل النمو الميكروبي على اللب مطحنة النفايات تيارات وتوصيف إمكانات التحلل الحيوي من الميكروبات مثقف
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Cite this Article

Mathews, S. L., Ayoub, A. S., Pawlak, J., Grunden, A. M. Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes. J. Vis. Exp. (82), e51373, doi:10.3791/51373 (2013).More

Mathews, S. L., Ayoub, A. S., Pawlak, J., Grunden, A. M. Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes. J. Vis. Exp. (82), e51373, doi:10.3791/51373 (2013).

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