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Medicine

Syngeneic Murine मेलेनोमा कोशिकाओं के इंजेक्शन फेफड़ों में उनकी Metastatic क्षमता का निर्धारण करने के लिए

Published: May 24, 2016 doi: 10.3791/54039

Materials

Name Company Catalog Number Comments
Trypsin Corning MT25052CV
RPMI 1640 Medium Corning MT15040CM
Phase Contraast Hemacytometer Hausser 02-671-54
Micro-Fine IV Insulin Syringes BD 14-829-1D
Sterile Alcohol Prep Pads Fisherbrand 22-363-750
Mouse Restrainer Braintree Scientific NC9999969 Restrainer choice depends on age/size of mice
Heating Pad Harry Schein NC0012697 Optional

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References

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  14. Terranova, V. P., Hic, S., Diflorio, R. M., Lyall, R. M. Tumor cell metastasis. Crit. Rev. Oncol. Hematol. 5 (2), 87-114 (1986).
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  17. Hart, I. The selection and characterization of an invasive variant of the B16 melanoma. Am J Pathol. 97 (3), 587-600 (1979).
  18. Overwijk, W. W., Restifo, N.P. B16 as a Mouse Model for Human Melanoma. Curr Protoc Immunol. 20 (1), (2001).
Syngeneic Murine मेलेनोमा कोशिकाओं के इंजेक्शन फेफड़ों में उनकी Metastatic क्षमता का निर्धारण करने के लिए
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Cite this Article

Timmons, J. J., Cohessy, S., Wong,More

Timmons, J. J., Cohessy, S., Wong, E. T. Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs. J. Vis. Exp. (111), e54039, doi:10.3791/54039 (2016).

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