JoVE Monthly Highlights: April 2019

1Journal of Visualized Experiments

Multiscale Sampling of a Heterogeneous Water/Metal Catalyst Interface using Density Functional Theory and Force-Field Molecular Dynamics

Cameron J. Bodenschatz*1, Xiaohong Zhang*1, Tianjun Xie*1, Jeremy Arvay1,2, Sapna Sarupria1, Rachel B. Getman1
* These authors contributed equally

The goal of the protocol presented here is to generate and sample trajectories of configurations of liquid water molecules around catalytic species on a flat transition metal surface. The sampled configurations can be used as starting structures in quantum mechanics-based methods.

Intense Pulsed Light for the Treatment of Dry Eye Owing to Meibomian Gland Dysfunction

Luca Vigo1, Giuseppe Giannaccare2, Stefano Sebastiani2, Marco Pellegrini2, Francesco Carones1

Dry eye disease is an increasingly common condition, which strongly impair patients' life quality. Recently, a new device employing intense pulsed light, specifically designed for the periocular area, has been shown to improve tear film stability and ocular discomfort symptoms in dry eye disease owing to meibomian gland dysfunction.

Radio Frequency Magnetron Sputtering of GdBa2Cu3O7δ/ La0.67Sr0.33MnO3 Quasi-bilayer Films on SrTiO3 (STO) Single-crystal Substrates

Ying Wang1,2, Zhen Li1,2, YongSheng Liu1, Yijie Li2, Linfei Liu2, Da Xu2, Xiaojing Luo1, Tian Gao1,3, Yanyan Zhu1,4, Luozeng Zhou3, Jianming Xu3

Here, we present a protocol to grow LSMO nanoparticles and (Gd) BCO films on (001) SrTiO3 (STO) single-crystal substrates by radio frequency (RF)-sputtering.

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Yena Kim1,2, Ji Hyeon Ju1,2,3

We propose a protocol that shows how to differentiate induced pluripotent stem cell-derived keratinocytes and fibroblasts and generate a 3D skin organoid, using these keratinocytes and fibroblasts. This protocol contains an additional step of generating a humanized mice model. The technique presented here will improve dermatologic research.