Journal Monthly Highlights: June 2019

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1JoVE Content Production

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

Ami Maruyama1,2, Kenjiro Kami3, Kazunori Sasaki3, Hajime Sato3, Yuzo Sato1,2, Katsuya Tsuchihara4, Hideki Makinoshima1,2,4
1Shonai Regional Industry Promotion Center, 2Tsuruoka Metabolomics Laboratory, National Cancer Center, 3Human Metabolome Technologies, Inc, 4Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center

The purpose of this article is to describe a protocol for extraction of aqueous metabolites from cultured adherent cells for metabolomic analysis, particularly, capillary electrophoresis-mass spectrometry.

Calculating Heart Rate Variability from ECG Data from Youth with Cerebral Palsy During Active Video Game Sessions

Corey Landis1, Margaret E. O'Neil2,3, Andrew Finnegan2,3, Patricia A. Shewokis1,3,4
1School of Biomedical Engineering, Science and Health Systems, Drexel University, 2Department of Physical Therapy and Rehabilitation Sciences, Drexel University, 3College of Nursing and Health Professions, Drexel University, 4Department of Nutrition Sciences, Drexel University

This protocol describes a method for calculating Heart Rate Variability (HRV) from electrocardiogram (ECG) waveforms. Waveforms from continuous heart rate (HR) recordings during active video game (AVG) sessions were used to measure the aerobic performance of youth with cerebral palsy (CP).

High Resolution Physical Characterization of Single Metallic Nanoparticles

Jessica Ettedgui1,2, Jacob Forstater1,2, Joseph W. Robertson1, John J. Kasianowicz1,3
1Physical Measurement Laboratory, National Institute of Standards and Technology, 2Department of Chemical Engineering, Columbia University, 3Department of Applied Physics and Applied Math, Columbia University

Here, we present a protocol to detect discrete metal oxygen clusters, polyoxometalates (POMs), at the single molecule limit using a biological nanopore-based electronic platform. The method provides a complementary approach to traditional analytical chemistry tools used in the study of these molecules.

Simultaneous Eye Tracking and Single-Neuron Recordings in Human Epilepsy Patients

Shuo Wang1, Nand Chandravadia2, Adam N. Mamelak2, Ueli Rutishauser2,3,4
1Department of Chemical and Biomedical Engineering, and Rockefeller Neuroscience Institute, West Virginia University, 2Departments of Neurosurgery and Neurology, Cedars-Sinai Medical Center, 3Center for Neural Science and Medicine, Department of Biomedical Sciences, Cedars-Sinai Medical Center, 4Division of Biology and Biological Engineering, California Institute of Technology

We describe a method to conduct single-neuron recordings with simultaneous eye tracking in humans. We demonstrate the utility of this method and illustrate how we used this approach to obtain neurons in the human medial temporal lobe that encode targets of a visual search.

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