Measuring Skeletal Muscle Thermogenesis in Mice and Rats

Summary

Mice and rats are surgically implanted with remote temperature transponders and then habituated to the testing environment and procedure. Changes in muscle temperature are measured in response to pharmacological or contextual stimuli in the home cage or during prescribed physical activity (i.e., treadmill walking at a constant speed).

Abstract

Skeletal muscle thermogenesis provides a potential avenue for better understanding metabolic homeostasis and the mechanisms underlying energy expenditure. Surprisingly little evidence is available to link the neural, myocellular, and molecular mechanisms of thermogenesis directly to measurable changes in muscle temperature. This paper describes a method in which temperature transponders are utilized to retrieve direct measurements of mouse and rat skeletal muscle temperature.

Remote transponders are surgically implanted within the muscle of mice and rats, and the animals are given time to recover. Mice and rats must then be repeatedly habituated to the testing environment and procedure. Changes in muscle temperature are measured in response to pharmacological or contextual stimuli in the home cage. Muscle temperature can also be measured during prescribed physical activity (i.e., treadmill walking at a constant speed) to factor out changes in activity as contributors to the changes in muscle temperature induced by these stimuli.

This method has been successfully used to elucidate mechanisms underlying muscle thermogenic control at the level of the brain, sympathetic nervous system, and skeletal muscle. Provided are demonstrations of this success using predator odor (PO; ferret odor) as a contextual stimulus and injections of oxytocin (Oxt) as a pharmacological stimulus, where predator odor induces muscle thermogenesis, and Oxt suppresses muscle temperature. Thus, these datasets display the efficacy of this method in detecting rapid changes in muscle temperature.

Introduction

Within metabolic research, the examination of skeletal muscle thermogenesis is a promising new avenue for probing body weight homeostasis. The published literature supports the idea that the thermogenic responses of one of the body's largest organ systems—the skeletal muscle—provide an avenue for increasing energy expenditure and other metabolic effects, thereby effectively rebalancing systems within diseases such as obesity^1,2,3. If the muscle can be considered a thermogenic organ, studies must utilize a practical methodology to study thermogenic changes within this organ. The desire to understand the endothermic impact of skeletal muscles and the utility of this methodology for studying non-shivering muscle thermogenesis are not specific to metabolic studies. Disciplines including evolution⁴, comparative physiology⁵, and ecophysiology^6,7 have shown a vested interest in understanding the ways in which muscle thermogenesis may contribute to endothermy and how this mechanism adapts to the environment. The presented protocol provides the critical methods necessary to address these questions.

The provided method can be utilized in the assessment of both contextual and pharmacological stimuli modulation of muscle temperature, including the unique technique of providing predator odor (PO) to shift the context to replicate predator threat. Prior reports have demonstrated the ability of PO to rapidly induce a sizable increase in muscle thermogenesis⁸. Moreover, pharmacological stimuli can also alter muscle temperature. This has been demonstrated in the context of PO-induced muscle thermogenesis, where pharmacological blockade of peripheral β-adrenergic receptors, using nadolol, inhibited the ability of PO to induce muscle thermogenesis without significantly affecting contractile thermogenesis during treadmill walking⁸. Central administration of melanocortin receptor agonists in rats has also been used to discern brain mechanisms altering thermogenesis^9,10.

Provided here is a preliminary investigation of the ability of the neurohormone oxytocin (Oxt) to alter muscle thermogenesis in mice. Similar to predator threat, social encounters with a same-sex conspecific increase body temperature, a phenomenon referred to as social hyperthermia¹¹. Given the relevance of Oxt to social behavior¹², it has been speculated that Oxt is a mediator of social hyperthermia in mice. Indeed, an oxytocin receptor antagonist decreases social hyperthermia in mice¹¹, and mouse pups lacking Oxt show deficits in behavioral and physiological aspects of thermoregulation, including thermogenesis¹³. Given that Harshaw et al. (2021) did not find evidence supporting β3 adrenergic receptor-dependent brown adipose tissue (BAT) thermogenesis with social hyperthermia¹¹, it has been posited that social hyperthermia may be driven by Oxt's induction of muscle thermogenesis.

To measure skeletal muscle thermogenesis, the following protocol uses the implantation of preprogrammed IPTT-300 transponders adjacent to the muscle of interest within a mouse or rat^8,10,14,15. These transponders are glass-encapsulated microchips that are read using corresponding transponder readers. Little to no research has utilized this technology in this capacity, though studies have suggested a need for the specificity provided by this method^16,17. Previous investigations have shown the reliability of this method and a variety of ways in which temperature transponders can be used in comparison with other temperature-testing methods¹⁸ or in conjunction with surgical methods (e.g., cannulation¹⁹). However, studies of this nature rely on different strategic placements to measure overall body temperature^20,21,22 or specified tissues such as BAT^23,24,25.

Rather than measuring temperature from these locations or while using ear or rectal thermometers²⁶, the method described here provides specificity for the muscle of interest. The ability to target a site by directly implanting transponders adjacent to the muscles of interest is more effective for probing muscle thermogenesis specifically. It provides a new avenue in addition to those provided by surface infrared thermometry^27,28 or cutaneous temperature measurements via thermocouple²⁹. Furthermore, the data provided through this method offer a range of avenues of research, avoiding the need for large, expensive, high-tech equipment and software such as infrared thermography^30,31,32.

This method has been successfully used to measure temperature in the quadriceps and gastrocnemius, either unilaterally or bilaterally. This method has also been effective in conjunction with stereotaxic surgery^14,15. Within ~7-10 cm of the transponder limb, portable transponder readers (DAS-8027/DAS-7007R) are used to scan, measure, and display the temperature. This distance has been critical and valuable to prior investigations^8,9,10 because it minimizes potential stressors and temperature-altering variables such as animal handling during the testing procedures. Using timers, measurements can then be recorded and collected over a period of time without direct interaction with the animals.

To further minimize the disturbance of mice during testing, this method describes the assembly and use of risers made of PVC piping to give the experimenter access to the bottom of the home cages during testing. Using the risers in tandem with the digital reader, temperature measurements of the transponder limb can be made without any animal interaction after the stimulus is placed. At a minimal cost, this method can be used in conjunction with pharmacological and contextual stimuli, making it quite accessible for researchers. Additionally, this method can be employed with a substantial number of subjects (~16 mice or ~12 rats) at a time, saving time in increasing the overall throughput for any research project.

Introduced in this method is a crafted mechanism for presenting odors to mice using stainless steel mesh tea infuser balls, from now on referred to as "tea balls". Though these tea balls are ideal for containing any odor material, in these studies, towels that served as in-cage bedding over 2-3 weeks for ferrets, a natural predator of mice and rats, are placed within each treatment tea ball. Each towel is cut into 5 cm x 5 cm squares. This aliquoting is also repeated with otherwise identical odorless control towels. Presenting these odors without a barrier (i.e., tea ball) led to mice shredding the fibers within their cages, increasing physical activity. This behavior was not as salient in rats. Tea balls provide a ventilated casing to the towel, giving full access to the odor while staying protected for the entirety of the experimental trial. These tea balls can be sanitized in accordance with animal use protocols, prepared, and introduced directly after surgery to begin habituating the animals to the structure along with the control stimulus. Mice can then live with the additional enrichment, decreasing the salience of the acute stimulus presentation.

Habituation to the presence of the tea ball is only one aspect of habituation that is critical to this method. The described habituation protocol also consists of repeated exposure to the testing procedure to normalize the testing environment (i.e., personnel, transportation and movement to the testing location, exposure to stimulus). This extended habituation minimizes nuanced responses from the animals and focuses measurements on the desired dependent variables (e.g., pharmacological or contextual stimuli). Previous assessment of this protocol has identified four trials as the minimum number of habituations necessary before temperature testing within home cages in rats⁸. If testing is separated by long periods (more than 2-3 weeks), the animals must be habituated again. For repeated habituation, a minimum of one to two trials are sufficient. However, if temperature tests are separated by more prolonged bouts of time, repeating more trials may be necessary.

In the continued effort to accustom mice and rats to the testing procedure, an acclimation period before stimulus presentation should be included in every experimental trial. This acclimation time is critical to rebalance temperature and activity after being shifted to the testing location. Rodents tend to have sharp temperature increases due to translocation. Acclimation should consist of a minimum of 1 h without interaction from the experimenter on the day of testing before any addition of a pharmacological agent or contextual stimuli. This is necessary each day of testing.

In the outlined home-cage temperature tests, mice have the free range of their home cage to roam in response to the tested stimulus. This can cause variable shifts in activity, impacting the accuracy of temperature readings and, therefore, the analysis of the thermogenic effects of the independent variable (e.g., pharmacological or contextual stimulus). In recognition of the potential changes in temperature due to activity level, a protocol is included below describing the use of temperature during treadmill walking. The published literature describes the successful use of this procedure in rats, and it is currently being employed with mice^8,10,14,15. Treadmill walking maintains a constant speed of activity for the testing subject. For this study, treadmills are strictly used to control activity level and, therefore, are set to the lowest available speed on the treadmill to promote walking for mice and a similarly low setting for rats.

The following procedure is outlined for the temperature measurement of unilateral gastrocnemius in mice and predator odor presentation. The design can be used in conjunction with pharmacological agents and is transferrable to rats and other skeletal muscle groups (i.e., quadriceps) in mice. For rats, transponders can be placed in the gastrocnemius bilaterally and in brown adipose tissue. Due to size and distance limitations, only one transponder can be used per mouse. Minor modifications (e.g., the removal of contextual stimuli) can be made to assess thermogenic responses to pharmacological agents.

Protocol

These methods can be applied to both rat and mouse models and were performed with institutional approval (Kent State University, IACUC Approval #359 and #340 CN 12-04). Prior to the implementation of the protocol, animals should be housed in conformance with the Guide for the Care and Use of Laboratory Animals.

1. Preparing the transponder reader

NOTE: Prior to use, the transponder reader must be set; the following steps only include the setting changes necessary for this study. This portion of the protocol is directly associated with the DAS-8027-IUS portable readers; other reader models should follow instructions provided by the manual to achieve programming results.

1. Set Audio Beep to OFF.
   1. Turn on the device by pressing the SCAN button and wait for the lighting to appear on the OLED display. Press and hold the BACK/MENU button to get to the menu screen.
   2. Using the NEXT/ENTER button, scroll through the options until OPERATIONAL SETUP. Here, toggle the up or down arrows to turn YES and open the operational submenu.
   3. Using the NEXT/ENTER button, scroll to AUDIO BEEP. As the default setting is ON, toggle the up or down arrows and change the setting to OFF.
   4. Press the NEXT/ENTER button to save this setting change.
2. Set Vibrate Upon Read to ON.
   1. Follow step 1.1 through step 1.2 or complete the next step directly after step 1.4.
   2. Using the NEXT/ENTER button, scroll to VIBRATE UPON READ. As the default setting is OFF, toggle the up and down arrows and change the setting to ON to feel, via vibration, when the reading has been completed regardless of being able to view the screen.

2. Program transponders

NOTE: Each implanted transponder should first be programmed with an animal identification (animal ID or transponder ID). This nomenclature can be used as secondary identification for the test subject (e.g., four digits for mouse strain abbreviation, location of transponder, and an additional three to four digits to indicate animal number). Programming can be completed days in advance of surgery while keeping the transponders sterile prior to surgery.

1. Enter the ID Code on the transponder.
   1. Apply a booster coil to the reader head—a specific accessory for model DAS 8027-IUS, which helps in the programming procedure.
   2. Using a gloved hand, place the transponder (within the applicator) into the booster coil.
   3. Turn on the device by pressing the SCAN button and wait for the OLED display to light up. Press and hold the BACK/MENU button to get to the menu screen.
   4. Using the NEXT/ENTER button, scroll through the options until WRITE TRANSPONDER ID. Here, toggle the up or down arrows to turn YES.
   5. Using the NEXT/ENTER button, toggle to ENTER ID CODE.
   6. Use the up and down arrow keys to scroll through numbers and letters. Press NEXT/ENTER after each character selection to move to the following character.
   7. When the ID code is complete, press SCAN to write the transponder.
   8. Remove the transponder from the booster coil and repeat as necessary. Check that the transponder reads temperature changes by warming the enclosed transponders between gloved hands and measuring using the temperature scanner.
      ​NOTE: AUTO MULTI WRITE and SEQUENTIAL COUNT settings can be set to ON to allow for multiple or sequential transponder programming during a session. Each transponder should be tested during programming.

3. Prepare "home cage balls"

1. Place 5 cm x 5 cm odorless/control towel into a tea ball.
2. Place these home cage balls in new home cages after surgery to begin habituating the animal to the method in which the contextual stimuli will be presented during testing. Replace these home cage balls every 2 weeks.

4. Surgery and postoperative care

1. Weigh and record the subjects' pre-surgery body weight. Using an induction chamber, provide anesthesia (e.g., 2-5% isoflurane) to the animal.
2. Using electric clippers, completely shave the hind limb. Administer analgesia (e.g., 5 mg/kg of ketoprofen, s.c.) in compliance with institutional guidelines.
   NOTE: Additional analgesia may be required if this procedure is combined with other surgical methods.
3. Clean the area with 70% alcohol (or commercially available sterile alcohol wipe) and povidone-iodine wash (or commercially available sterile, individually wrapped betadine swabs) alternating at least three times, ending with povidone-iodine.
4. Return the animal to the induction chamber, and anesthetize the animal to surgical levels. Then, set up the mouse in a face mask for continued exposure to anesthesia. Apply neomycin ophthalmic ointment to the eyes of the animal to prevent dryness while under anesthesia.
   NOTE: The procedure should not start until the mouse shows no evidence of pain reception (i.e., corneal reflex, tail pinch response, toe pinch reflex).
5. Using only surgical scissors, make a shallow cut through the skin on the right hind limb.
6. Moving parallel to the gastrocnemius, place the sharp edge of a preprogrammed and uncapped sterile transponder into the incision. Ensure that the green plunger faces up and is visible. Continue pushing the transponder applicator into the incision until the opening of the transponder applicator is no longer visible.
   NOTE: Do not accidentally press the green plunger on the transponder applicator during step 4.6. Premature discharge of the transponder will lead to improper placement.
7. Turn the applicator 180°, resulting in the green plunger facing down toward the mouse's limb, no longer visible to the experimenter. Push the transponder applicator into the final location. Once in ideal placement, adjacent to or partially enclosed in the gastrocnemius, push the green plunger, allowing the applicator's pressure to guide the investigator's hand back away from the mouse.
8. Using forceps, hold together the opened skin and place a wound clip with a sterile autoclip or sterile suture. If needed, use absorbable sutures prior to the sterile autoclip to close the fascia layer. Using the transponder-reader, check the temperature of the mouse muscle.
9. Remove the mouse from anesthesia and place it in a clean home cage placed atop a water-circulating heating pad set to low for recovery. Ensure that the home cage includes a tea ball with an odorless towel to begin habituation.
   NOTE: The mouse should awaken from surgery within 15 min. Food can be placed at the bottom of the cage for easy access during recovery days.
10. Postoperative care
    1. Record mouse weights and temperatures daily using a transponder-reader for at least 2 days after surgery or until mice regain or stabilize body weight.
    2. Administer non-narcotic analgesia (e.g., 5 mg/kg of ketoprofen, s.c.) once daily to the mice for at least 2 days post surgery, with additional doses provided as needed.
       NOTE: Mice and rats should fully recover within 5-8 days of surgery and can undergo habituation and testing procedures.

5. Testing preparation - home cage

1. Constructing risers
   NOTE: The below step is based on 194 mm x 181 mm x 398 mm mouse filter-topped cages. To fit larger cages (e.g., a rat home cage), the width will need to be adjusted.
   1. Cut the PVC pipe with a ratcheting PVC cutter into eight sections and assemble following Figure 1C. This will give an open tabletop structure that can hold approximately four cages. Make the desired number of risers.
2. Room setup
   1. Assign a location to each riser within the testing room. Separate the risers set to receive different contextual stimuli (i.e., odors) by a minimum of 2 m to avoid confounding variables.
      NOTE: Each mouse should have an assigned testing location within the testing room and on the physical risers as much as is feasible to avoid developing associations between different locations and thermogenic stimuli.
   2. Using magnetic strips, attach surgical sheets or gowns across the risers, creating a visual barrier between the researcher and the test subjects. Set this barrier to minimize temperature changes resulting from mouse activity when viewing experimenters moving toward the cage or around the testing room.
   3. (Optional) Place mirrors on the surface below the risers to ease viewing of the cage bottom during testing.
      NOTE: Risers can be sanitized through a cage wash system. Cloth or surgical sheets should be laundered prior to habituation and testing.
3. Tea ball preparation
   1. Prepare tea balls with control and PO towels (approximately 5 cm x 5 cm). To avoid cross-contamination, prepare control-odor tea balls first.
      NOTE: Predator-odor towels should be pathogen-tested prior to use. These towels should also be contained, and materials that interact with them should be immediately sanitized (i.e., cage wash), preventing exposure of the odor to other animals.

6. Temperature testing - home cage

NOTE: Animals need to be habituated to the entire testing procedure, excluding experimental contextual, or pharmacological stimuli. This should be completed a minimum of 4x before testing.

1. Transfer the animals to the prepared testing room. Place the animals in a preassigned location on the riser. This location should be the same throughout all habituation and testing procedures.
2. Remove the "home cage ball" from the mouse home cage and re-cover the cages with a cloth or surgical sheet. Allow the mice to acclimate to the testing space for 1-2 h.
3. After acclimation is complete, use the scanner to measure and record the baseline temperature of each subject. Avoid manipulating the cloth coverings during measurements.
   NOTE: Pharmacological agents can be applied here. Wait time post injection or application can be added as needed before testing. Recording a secondary baseline directly before testing is recommended after the addition of a pharmacological agent to monitor the response to pharmacological stimuli. If odor response is not being tested, temperature measurements of the mice can begin directly after injection. Randomization should be employed when providing any stimuli.
4. Uncover the cage and place the tea ball (control or PO) onto the floor of the home cage. Replace the cage lid and cloth covering.
5. Begin the stopwatch. Measure the temperatures of the test subjects in the same order of tea ball placement. Record temperatures and clock time of measurements following the desired time points.
6. When the experiment is complete, remove the treatment ball. Place the mice that received PO in a new home cage with the original "home cage ball". Return the "home cage ball" to the cage of the mice that received control odor. Transfer the mice to the housing location.
   ​NOTE: The above procedure can be translated to rat models within appropriately sized cages. Adjustments to the measurements suggested in Figure 1C may be required to allow for better access to the bottom of the home cage.

Figure 1: Transponders and home cage temperature testing. (A) Diagram of unilateral transponder placement for testing temperature in a mouse gastrocnemius. Once programmed and placed, the transponder-reader (DAS-8027-IUS, shown) can be used to measure temperature. (B) Left, photo of an open mesh stainless steel tea ball and a 5 cm x 5 cm towel. Right, enclosed tea ball, used to hold habituation and odor towels in home cage testing. (C) Schematic of risers constructed with PVC piping for home cage testing. (D) Workflow of home cage testing protocol. (E) Facility images of home cage testing area. Left, four mouse cages atop a riser. Magnetic strips are located on the adjacent wall, and magnets and surgical cloth are on the table. Right, covered mouse cages on risers. (A), (C), and (D) were created with Biorender.com. Please click here to view a larger version of this figure.

7. Temperature testing - treadmill walking

1. Assign each animal a treadmill as their assigned location for habituation and testing procedures.
2. Prepare the treadmills for testing, ensuring that the shockers are functional.
   NOTE: For treadmill walking, treadmills should be set at the lowest available pace that promotes continuous movement but not running for both habituation and testing. For the 1012M-2 Modular Enclosed Metabolic Treadmill, this is 5.2 m/min for mice and 7 m/min for rats. This pace may need to be adjusted based on the obesity of the subject. Shockers should be set to an intensity and repetition rate of 5.0.
3. Habituation
   1. Move the mice to the testing room. Allow mice 1-2 h to acclimate to the room transfer in their home cages.
   2. After acclimation, guide the mice to the opening of their assigned treadmill and close the treadmill. Start the belt, shocker, and stopwatch.
   3. Allow the mice to walk on the treadmills for 15 min, using shock stimulus as motivation for movement. Stop the test immediately if an animal remains on an active shocker for an extended period.
   4. After the test, remove the mice and return them to the home cages.
   5. Clean the treadmills using liquid detergent and water.
4. Testing
   1. Move the mice to the testing room. Allow the mice 1-2 h to acclimate to the room transfer in their home cages.
   2. Measure and record baseline temperature prior to moving the mouse to the treadmill.
      NOTE: For tests including pharmacological agents, apply or inject them here, following the schematic shown in Figure 2A. Wait time after injection can be added as needed before the mice are placed on the treadmill. Randomization should be employed when providing any stimuli.
   3. Place 5 cm x 5 cm squares of control or PO towels within the treadmill closest to the front of the treadmill. Adhere the towels to the ceiling of the treadmill or underneath for easy placement and removal.
   4. Guide the mice into the assigned treadmill. Turn on the treadmill belt and shocker.
   5. Start the stopwatch. Take measurements of the test subjects in the same order in which the mice were set up in the treadmills. Record the temperatures and clock time of the measurements following the desired time points.
      NOTE: Temperature can reliably be measured from outside the treadmill while a mouse is inside an enclosed treadmill during walking activity. For rats, the treadmill size and transponder-reader distance limitations may require an experimenter to keep the back of the treadmill open to insert the reader inside the treadmill, closer to the subject.
   6. When the test is complete, turn off the shockers and treadmills; return the mice to their home cages. Transfer the mice to the housing location.
   7. Clean the treadmills using liquid detergent and water, paying specific attention to remove any residual PO.
   8. When experiments are complete, euthanize the animals (e.g., using CO₂ inhalation), and visually confirm the transponder location.

Figure 2: Activity-controlled temperature testing. (A) Workflow of activity-controlled temperature testing with a pharmacological agent using treadmill walking. (B) Facility images of treadmills. Left, an image of full equipment setup. Right, a closer image of individual treadmills and shockers. (A) was created with Biorender.com. Please click here to view a larger version of this figure.

Results

Transponders were unilaterally implanted into the right gastrocnemius of ten 4-6-month-old, wild-type (WT) mice bred from the SF1-Cre strain (Tg(Nr5a1-cre)7Lowl/J, Strain #012462, C57BL/6J and FVB backgrounds; female N = 5; male N = 5). After recovery, the mice were habituated to a home cage temperature-testing procedure that did not include a contextual stimulus (e.g., PO). Temperature measurements using a transponder wand were recorded within their housing room and after transfer to the testing location. Mice were give...

Discussion

This temperature testing protocol provides the field with an avenue to measure skeletal muscle thermogenesis directly. This is critical as research delves into identifying the mechanisms underlying muscle thermogenesis³³. The method provides two cost-effective protocols for measuring skeletal muscle thermogenesis under contextual and pharmacological conditions. This protocol emphasizes the importance of both habituation and acclimation within these procedures. Habituation is used to repeatedly int...

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
1012M-2 Modular Enclosed Metabolic Treadmill for Mice, 2 Lanes w/ Shock	Columbus Instruments
1012R-2 Modular Enclosed Metabolic Treadmill for Rats, 2 Lanes w/ Shock	Columbus Instruments
1-1/4 in. Ratcheting PVC Cutter	BrassCraft
1 mL Syringes	Fisher Scientific	BD 309659
Betadine Swabs	Fisher Scientific	19-898-945
Booster Coil	BioMedic Data Systems		Transponder Accessory
Electric Clippers	Andis		40 Ultraedge Clipper Blade
Flexible Mirror Sheets	Amazon		Self Adhesive Non Glass Mirror Tiles
Forceps	Fisher Scientific	89259-940
Heating Pad
Induction Chamber (isoflurane)	Kent Scientific	VetFlo-0730	3.0 L Low Cost Chambers for Traditional Vaporizers
Ketoprophen	Med-Vet Intl.	RXKETO-50
Magnetic Strips	Amazon
Magnets	Amazon		DIYMAG Magnetic Hooks 40lbs
Needles	Med-Vet Intl.	26400
Neomycin/Polymixin/Bacitracin with Hydrocortisone Ophthalmic Ointment, 3.5 g	Med-Vet Intl.	RXNPB-HC
Oasis Absorbable Suture	Med-Vet Intl.	MV-H821-V
Predator (Ferret) Odor Towels	Marshall BioResources
PVC pipe
Reflex Wound Clip Remover	CellPoint Scientific
Reflex Wound Clip, 7 mm (mouse)	CellPoint Scientific
Reflex Wound Clip, 9 mm (rat)	CellPoint Scientific
Srerile Autoclip, 7 mm (mouse)	CellPoint Scientific		Wound Clip Applier (mouse)
Stainless Strainers Interval Seasonings Tea Infuser	Amazon
Sterile Autoclip, 9 mm (rat)	CellPoint Scientific		Wound Clip Applier (rat)
Sterile Saline	Med-Vet Intl.	RX0.9NACL-10
Surgical Scissors	Fisher Scientific	08-951-5
Surgical Sheets
Towels (Control/Habituation)	Amazon		100% Cotton Towels, white
Transponders	BioMedic Data Systems		Model: IPTT-300
Transponders Reader	BioMedic Data Systems		Model: DAS-8027-IUS/ DAS-7007R
Versaclean	Fisher Scientific	18-200-700	liquid detergent
Webcol Alcohol Preps	Covidien	22-246-073
Wedge pieces for PVC pipe