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Biology

Trypsinizing和传代的哺乳动物细胞

doi: 10.3791/755 Published: June 12, 2008

Summary

当细胞达到汇合,他们必须传代或传代。此影片将展示壁和悬浮细胞传代的过程。

Abstract

当细胞达到汇合,他们必须传代或传代。亚文化的融合细胞在有丝分裂指数降低,并最终在细胞死亡的结果失败。在传代培养的第一步是分离细胞从原代培养血管的胰蛋白酶或机械手段的表面。由此产生的细胞悬液,然后再细分,或reseeded到新鲜的文化,。检查中学文化的增长和美联储定期,和随后可能会传代,大专文化。随细胞株传代细胞之间的时间取决于增长率。

Protocol

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这个实验方法的完整的文本协议是可在细胞生物学中的电流协议。

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Trypsinizing和传代的哺乳动物细胞
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Cite this Article

Ricardo, R., Phelan, K. Trypsinizing and Subculturing Mammalian Cells. J. Vis. Exp. (16), e755, doi:10.3791/755 (2008).More

Ricardo, R., Phelan, K. Trypsinizing and Subculturing Mammalian Cells. J. Vis. Exp. (16), e755, doi:10.3791/755 (2008).

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