Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Murray C. Killingsworth; Yuri V. Bobryshev

doi:10.3791/54307

Dergi

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Biyomühendislik

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Kuantum Nokta Nanopartiküller kullanma Korelatif Işık- ve Elektron Mikroskobu

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

JoVE Journal
Biyomühendislik

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JoVE Journal Biyomühendislik

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Kuantum Nokta Nanopartiküller kullanma Korelatif Işık- ve Elektron Mikroskobu

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9,689 Views

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11:16 min

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August 07, 2016

DOI:

10.3791/54307-v

DOI:

10.3791/54307-v

•

11:16 min

August 07, 2016

•

9665 Views

Murray C. Killingsworth^1,2,3,4, Yuri V. Bobryshev^3,4,5

¹South Western Sydney Clinical School, Faculty of Medicine,University of New South Wales Australia ²School of Medicine,Western Sydney University ³Correlative Microscopy Group,Ingham Institute for Applied Medical Research ⁴Electron Microscopy Laboratory, Department of Anatomical Pathology, Sydney South West Pathology Service,New South Wales Health Pathology ⁵School of Medical Sciences, Faculty of Medicine,University of New South Wales Australia

Özet

A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of epoxy embedded human pathology tissue. We employ commercial antibody fragment conjugated QDs that are visualized by widefield fluorescence light microscopy and transmission electron microscopy.