Canlı Hücrelerde eIF4E-eIF4G Etkileşimi Ölçülerek eIF4F Montajlarını İzleme
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May 01, 2020
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Özet
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Burada, kullanıcının eIF4F karmaşık dinamiklerinin ilaç kaynaklı pertürbasyonunu tarama formatlarında değerlendirmesini sağlayacak canlı hücrelerde eIF4E-eIF4G etkileşimini ölçmek için bir protokol sunuyoruz.
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Cite this Article
Frosi, Y., Ramlan, S. R., Brown, C. J. Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells. J. Vis. Exp. (159), e60850, doi:10.3791/60850 (2020).
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