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Linearisatie van de Bradford Protein Assay
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Linearisatie van de Bradford Protein Assay

Article DOI: 10.3791/1918-v • 06:35 min • April 12th, 2010
April 12th, 2010 •
1, 1
1Department of Biochemistry, Tel Aviv University

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De nauwkeurigheid en gevoeligheid van eiwit vaststelling door de snelle en handige Bradford assay in gevaar wordt gebracht door intrinsieke niet-lineariteit. We tonen een eenvoudige procedure die linearisatie verhoogt de nauwkeurigheid, verbetert de gevoeligheid van de test ongeveer een factor 10, en vermindert de inmenging van wasmiddelen.

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Cellular Biology Bradford eiwit assay eiwit kwantificering Coomassie briljant blauw
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