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Journal / Biology / Imaging of Mitochondrial Peroxide in Living Yeast … / Preparation of Yeast Cells for Imaging,…
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Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection (Video) | JoVE
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Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection (Video) | JoVE

Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7

Article DOI: 10.3791/200471-v • 02:33 min • June 2nd, 2023
June 2nd, 2023 •
1, 1,2, 2, 1,2, 1,2
1Department of Pathology and Cell Biology, Columbia University Irving Medical Center, 2Confocal and Specialized Microscopy Shared Resource in the Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center

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MtHyPer7 ratiometrisk biosensor mitokondrielt peroksid levende gjærceller mitokondriell dysfunksjon nevrodegenerative lidelser muskel- og skjelettlidelser kreft aldring genetisk kodet biosensor minimalt invasiv hydrogenperoksid (H2O2) mitokondrier-målrettet HyPer7 (mtHyPer7) sirkulært permutert fluorescerende protein OxyR-proteindomene CRISPR-Cas9-system markørfritt system gjærgenomintegrasjon spinningsdisk konfokalmikroskopsystem kvantitativ analyse oksidativ Stress Spatiotemporal informasjon
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