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Bestimmung der Na+-K+-ATPase- und Ca2+-MG2+-ATPase-Aktivität in Salidrosid-behandelten MCF-7-Zellen

Salidroside's Mechanism in Suppressing MCF-7 Cell Migration and Proliferation

Article DOI: 10.3791/200538-v • 00:54 min • June 9th, 2023
June 9th, 2023 •
1, 1, 1, 1, 1, 1, 1, 2
1Pathology Department, Suining Central Hospital, 2General Surgery Day Ward, Department of General Surgery, the Third People's Hospital of Chengdu & the Affiliated Hospital of Southwest Jiaotong University

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Salidrosid pharmakologische Wirkung molekularer Mechanismus Hemmung der MCF-7-Zellproliferation Hemmung der MCF-7-Zellmigration antikarzinogen antihypoxisch entzündungshemmend PI3K-AKT-HIF-1α-FoxO1-Signalweg CCK-8-Assay Zell-Scratch-Assay Migrations-Assay Matrigel-Invasions-Assay Zellapoptose-Assay Zellzyklus-Assay reaktive Sauerstoffspezies (ROS) Ca2+ Na+-K+-ATPase-Aktivität Proteinexpressionsniveaus Genexpressionsniveaus Western-Blot-Analyse QRT-PCR-Analyse
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