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Journal / Cancer Research / Isolation of Nuclei for Multiome Sequencing in… / 从人胰腺肿瘤细胞中分离细胞核进行多组测序…
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Cancer Research

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JoVE Journal Cancer Research
从人胰腺肿瘤细胞中分离细胞核进行多组测序
Using the E1A Minigene Tool to Study mRNA Splicing Changes
Journal (Cancer Research)
Using the E1A Minigene Tool to Study mRNA Splicing Changes
Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract (ECME)-based Three-dimensional System
Journal (Cancer Research)
Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract (ECME)-based Three-dimensional System
 
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从人胰腺肿瘤细胞中分离细胞核进行多组测序

Isolation of Nuclei for Multiome Sequencing in Solid Tumor Specimen

Article DOI: 10.3791/200649-v • 02:13 min • October 13th, 2023
October 13th, 2023 •
1, 1, 1, 1, 1, 1
1Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Stanford University

Tags

优化的细胞核分离、新鲜肿瘤标本、冷冻肿瘤标本、多组学测序、单细胞 RNA 测序、ATAC 测序、肿瘤细胞异质性、转化癌症研究、测序数据质量、消化条件优化、细胞产量最大化、实体瘤结缔增生性基质、细胞释放、脆性细胞、异质细胞类型、总细胞群支持、核分离条件优化、裂解时间、试剂类型/比率、10x 基因组学多组测序平台
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