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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Cancer Research
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JoVE Journal Cancer Research
Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

DOI:
10.3791/62181-v

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10:25 min

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April 22, 2021

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, ,
1Faculdade de Ciências Farmacêuticas,Universidade Estadual de Campinas

Chapters

  • 00:05Introduction
  • 00:35Cell Transfection
  • 01:58Cell Treatment and Collection
  • 02:55RNA Extraction and cDNA Synthesis
  • 05:44pMTE1A Minigene PCR and Gel Analysis
  • 08:13Results: Effects of Cisplatin and Paclitaxel on Minigene E1A Splicing Pattern
  • 09:27Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents a rapid and useful tool for evaluating the role of a protein with uncharacterized function in alternative splicing regulation after chemotherapeutic treatment.

Tags

E1A MinigeneMRNA SplicingHEK 293 CellsCell CultureTransfectionRNA ExtractionAlternative SplicingGene ExpressionChemotherapeuticsNek4

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