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Journal / Neuroscience / DREADDs Technology and Automated Blood Sampling / Stereotaksisk levering af adeno-associerede vira…
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Neuroscience

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JoVE Journal Neuroscience
Stereotaksisk levering af adeno-associerede vira til en bestemt cellepopulation forbundet med seriel automatiseret blodindsamling
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Stereotaksisk levering af adeno-associerede vira til en bestemt cellepopulation forbundet med seriel automatiseret blodindsamling

DREADDs Technology and Automated Blood Sampling

Article DOI: 10.3791/200684-v • 02:47 min • August 25th, 2023
August 25th, 2023 •
1, 1,2, 1,2, 1,2
1Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, 2Mouse Metabolic Phenotyping Center-Live, University of Michigan, Ann Arbor

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Fjernneuronal aktivering automatiseret blodprøvetagning cirkulerende luteiniserende hormon mus hypothalamus-hypofysekontrol reproduktion LH-frigivelse modulation input neuronale populationer miljøstress måleteknikker langsigtet træning manipulationsstress tilbageholdenhed forskningsspørgsmål designerreceptor udelukkende aktiveret af designerlægemidler (DREADD'er) adeno-associeret virus (AAV) vektorer stereotaksisk kirurgisk protokol halspulsårenylering jugularvene Kanylering CULEX automatiseret blodprøvetagningssystem clozapin-N-oxid intravenøs injektion
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