Corneoscleral Button Isolation: A Surgical Procedure to Obtain Corneoscleral Button from Porcine Eye

The anterior portion of the eye is covered by the cornea - a transparent layer that allows the transmission of light rays. The cornea extends into the sclera - a white protective tissue around the eye. The part of the eye that consists of the cornea surrounded by a thin ring of the sclera is the corneoscleral button.

To excise the corneoscleral button, place an intact porcine eye on a Petri dish. Remove the conjunctiva - a mucilaginous covering around the sclera - along with the surrounding extraocular muscles to access the scleral cover. Treat the eye with a suitable disinfectant solution to remove any contaminating microorganisms from the eye's surface. 

Wash off the disinfectant with a buffer solution. Transfer the disinfected eye to a fresh culture dish. Make an incision on the sclera near the corneal boundary. Using this opening, make a complete circumferential cut to divide the eye into two fragments.

Visualize the interior side of the frontal segment to locate the uveal coat attached to the sclera. This tissue consists of pigmented iris, a ring-shaped ciliary body, and a portion of the vascular choroid layer.  Detach the uvea to visualize the corneoscleral layer. Store the button in a sterile buffer solution for further assays.