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Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients

Article DOI: 10.3791/2830-v • 13:50 min • June 11th, 2011
June 11th, 2011 •
1, 1, 1, 1, 1
1Department of Medicine, Surgery and Dentistry, Clinic of Infectious Diseases, San Paolo Hospital University of Milan, Italy

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Our experiment will show how to perform a sequencing analysis of bacterial species translocating in peripheral blood of HIV positive patients.

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Sequencing Bacterial Microflora Peripheral Blood HIV-infected Patients Gastrointestinal Tract Commensal Microbes Mucosal Immune System Microbiota Immune Abnormalities Breach In The Gastrointestinal Barrier Mucosal Immunity Systemic Circulation Bacterial Bioproducts Lipopolysaccharide Bacterial DNA Fragments Systemic Immune Activation Microbial Translocation HIV/AIDS Immunopathogenesis Response To Therapy Characterise The Composition Of Bacteria Translocating In Peripheral Blood PCR Reaction Panbacteric 16S Ribosomal Gene Sequencing Analysis
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