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前溶瘤病毒的活组织的体内感染
JoVE Journal
Medicine
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JoVE Journal Medicine
Ex Vivo Infection of Live Tissue with Oncolytic Viruses
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前溶瘤病毒的活组织的体内感染

DOI:
10.3791/2854-v

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12:08 min

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June 25, 2011

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, , , ,
1Center for Innovative Cancer Research,Ottawa Hospital Research Institute (OHRI)

Chapters

  • 00:05Title
  • 02:00Tissue Processing
  • 03:00Assessment of Tissue Viability
  • 04:48Visualization of GFP Transgene Expression by Fluorescence Microscopy
  • 05:50Visualization of Luciferase Expression using an In Vivo Imaging System (IVIS)
  • 06:33Assessing Viral Titers by Plaque Assay
  • 09:52Viability and Infectability of Patient Tissue Samples
  • 11:07Conclusion

Summary

Automatic Translation

Automatic Translation

溶瘤病毒是有希望的癌症治疗。的能力，以确定的活组织标本从患者治疗前infectability，是一个独特的优势，这种治疗方法。本协议描述了如何处理组织<em>体外</em>溶瘤病毒和随后的病毒定量的感染。

Tags

Ex Vivo InfectionOncolytic VirusesClinical TrialsHSVReovirusVaccinia OVsGenetically ModifiedReporter TransgenesMicroscopyBio-luminescence ImagingOncolytic VirotherapyViral ReplicationAbortive InfectionProductive InfectionProtocolAlamar Blue Metabolic DyeGFPFirefly Luciferase Detection

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