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Journal / Bioengineering / Tracking Hypoxische Signalering binnen…
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Bioengineering

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JoVE Journal Bioengineering
Tracking Hypoxische Signalering binnen Encapsulated Cell Aggregaten
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Tracking Hypoxische Signalering binnen Encapsulated Cell Aggregaten

Article DOI: 10.3791/3521-v • 09:14 min • December 16th, 2011
December 16th, 2011 •
1, 1, 2
1Biomedical Engineering Program, University of South Carolina, 2Chemical Engineering Department, University of South Carolina

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Een methode voor het foto-inkapseling van cellen in een verknoopt PEG hydrogel wordt beschreven. Hypoxische signalering binnen ingekapseld murine insulinoom (MIN6) aggregaten is gevolgd met behulp van een fluorescerende marker-systeem. Dit systeem maakt serie onderzoek van de cellen binnen een hydrogel steiger en correlatie van hypoxische signalering met veranderingen in de cel fenotype.

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Bioengineering Cell inkapseling PEG cel aggregatie hypoxie insuline secretie fluorescerende beeldvorming
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