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Analyse de l'expression de mammifères Linker-histones sous-types
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Analyse de l'expression de mammifères Linker-histones sous-types

Article DOI: 10.3791/3577-v • 14:40 min • March 19th, 2012
March 19th, 2012 •
1, 1, 1, 1
1School of Biology and the Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology

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Nous décrivons un ensemble de tests pour analyser les niveaux d'expression des histones H1. ARNm de gènes H1 individuels sont mesurés quantitativement par transcription inverse amorce aléatoire basé suivie par PCR en temps réel, alors que la quantification des protéines histones H1 de est obtenue par une analyse HPLC.

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Génétique Numéro 61 histones H1 sous-types H1 histones chromatine RT-PCR HPLC l'expression des gènes
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