/
/
Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 02:38Dissection
- 04:51Tissue Analysis: Total Protein and Final Normalization of Tyrosine Hydroxylase and Dopamine Active Transporter Results
- 08:12Results: Tyrosine Hydroxylase Phosphorylation in Rat Substantia Nigra and Ventral Tegmental Samples
- 09:00Conclusion
Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in rodents.
Tags
Dopamine RegulationNeurotransmitterCNSLocomotor ActivityReward-related BehaviorMolecular DeficienciesNigrostriatal PathwayMesoaccumbens PathwayStriatumNucleus AccumbensTyrosine Hydroxylase ProteinTH PhosphorylationDopamine Transporter ProteinVesicular Monoamine Transporter 2 ProteinPost-translational ModificationsMidbrain
