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Journal
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Immunology and Infection
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耐高温溶菌酶的定向进化的一个新的筛选方法
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
Please note that all translations are automatically generated.
Click here for the English version.
耐高温溶菌酶的定向进化的一个新的筛选方法
DOI:
10.3791/4216-v
•
13:30 min
•
November 07, 2012
•
Ryan D. Heselpoth
,
Daniel C. Nelson
1
Institute for Bioscience and Biotechnology Research
,
University of Maryland
Chapters
00:05
Title
01:25
Determining Optimal Heating Conditions
06:15
Generating the Mutant Library
08:44
Soluble Lysate Heat Treatment
10:49
Results: Directed Evolution to Increase the Thermostability of PlyC
13:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
热稳定性工程领域的一种新型的定向进化方法的具体开发,从而验证溶菌酵素。随机诱变只有一轮中,演进式溶菌酶,PlyC 29C3,显示后大于两倍的残留活性相比升高的温度下温育后的野生型蛋白。
Tags
Directed Evolution
Thermostable Bacteriolytic Enzymes
Screening Method
Molecular Engineering
Protein Properties
Rational-based Approaches
Variants
Structural Stability
Endolysins
Bacteriophage
Peptidoglycan
Cell Wall
Lysis
PlyC Endolysin
PlyCA Subunit
PlyCB Subunit
Group A Streptococci (GAS)
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