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JoVE Journal
Immunology and Infection

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An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
 

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

Article DOI: 10.3791/62654-v
May 6th, 2021

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Summary May 6th, 2021

This manuscript describes a detailed protocol for differentiation of human embryonic stem cells (hESCs) into functional hepatocyte-like cells (HLCs) by continuously supplementing Activin A and CHIR99021 during hESC differentiation into definitive endoderm (DE).

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