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An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

DOI:
10.3791/62654-v

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08:05 min

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May 06, 2021

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, , , ,
1Stem Cell Research Center,Shantou University Medical College, 2The Center for Reproductive Medicine,Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology,Shantou University Medical College

Chapters

  • 00:04Introduction
  • 00:45Stem Cell Maintenance
  • 02:28Stem Cell Passage and Differentiation and Definitive Endoderm Formation
  • 03:46Differentiation of Hepatic Progenitor Cells and Hepatocyte Differentiation
  • 05:12Results: Directed Hepatocyte-like Cell Induction from Human Embryonic Stem Cells
  • 07:14Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript describes a detailed protocol for differentiation of human embryonic stem cells (hESCs) into functional hepatocyte-like cells (HLCs) by continuously supplementing Activin A and CHIR99021 during hESC differentiation into definitive endoderm (DE).

Tags

Keywords: Human Embryonic Stem CellsHepatocyte-like CellsDifferentiationDefinitive EndodermFOXA2SOX17GATA4CXCR4FOXA1MatrigelMTESRActivin ACHIR99021Cell CultureDisease ModelingDrug Screening

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