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提取组织抗原的功能分析

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Biology

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JoVE Journal Biology

Extraction of Tissue Antigens for Functional Assays

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提取组织抗原的功能分析

DOI:

•

08:32 min

•

September 10, 2012

•

Andra Necula, Rochna Chand, Batool Albatat, Stuart I. Mannering²

¹Immunology and Diabetes Unit,St. Vincent’s Institute of Medical Research, ²Department of Medicine,University of Melbourne

Chapters

00:05Title
01:13Preparing Spleen Tissue
01:57Preparing Human Islets for Storage
02:43Preparing Tissue Extract
04:47Freeze Drying Extracts
06:17Results: Functional T cell Assays of Extracts
08:08Conclusion

Summary

Automatic Translation

一个简单的协议，用于制备的人体组织的提取物被用作功能性T细胞分析中的源的抗原进行说明。此方法允许要被测量的组织来源的抗原的T细胞的反应，以<em在体外</em>。

Tags

Functional Assays Adaptive Immune Response B Cells T Cells Autoimmune Diseases Cancer Type 1 Diabetes Protein Antigens Functional Compatibility Protein Concentration Lyophilization Immune Cells Cytokine Production Proliferation PBMC

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